GASTRIC FUNCTION--TRH AND 5HT IN CAUDAL RAPHE CONTROL

胃功能--尾中缝控制中的TRH和5HT

• 批准号: 2016746
• 负责人: ROBERT L STEPHENS
• 金额: $ 16.13万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-15 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2016746
• 关键词: laboratory rat; medulla oblongata; membrane potentials; neuroendocrine system; serotonin; serotonin inhibitor; serotonin receptor; stomach motility /pressure; thyrotropin releasing hormone; vagus nerve

DESCRIPTION: TRH and 5HT are colocalized in the projections of caudal raphe nuclei onto dorsal vagal complex (DVC) nuclei. TRH and 5HT synergism appears to be involved in the vagal control of gastric function. Microinjections of selective 5HT antagonists will be performed in DVC in order to identify the 5HT receptor subtype involved in the synergistic response. Serotonin release in the dorsal medulla of fed and fasting animals will be compared to document that serotonin release by caudal raphe nuclei projections plays a key role in enhanced acid secretion and motility after a meal. A possible role of excitatory amino acids and substance P as neurotransmitters in the vagal afferents which mediate gastric stimulatory response after a meal will be examined with the use of the agonists and the antagonists of substance P and glutamate. Studies will also be performed to identify DVC neurons (using extracellular recordings) which are activated by gastric distension. Effects and synergistic interactions of TRH and 5HT microinjection directly onto solitary nucleus or DVC nuclei on the "gastric responsive DVC neurons" will be examined.

描述：TRH和5 HT共定位于尾侧核的投射， 中缝核到迷走神经背侧复合体（DVC）核。 TRH和5 HT 迷走神经对胃电活动的控制可能与协同作用有关。 功能将进行选择性5 HT拮抗剂的微量注射 在DVC中，为了鉴定参与DVC的5 HT受体亚型， 协同反应。 大鼠足背髓质5-羟色胺释放的研究 和禁食的动物进行比较，记录血清素的释放， 中缝尾核投射在增强酸性中起关键作用 餐后的分泌和运动。 兴奋性的可能作用 氨基酸和P物质作为迷走神经传入的神经递质 其在餐后介导胃刺激反应 使用P物质的激动剂和拮抗剂， 谷氨酸盐。 还将进行研究以识别DVC神经元（使用 细胞外记录），其由胃扩张激活。 TRH和5-HT微量注射的效应及其协同作用 在“胃反应性”上直接投射到孤核或DVC核 将检查DVC神经元。

项目成果

5-CT or DOI augments TRH analog-induced gastric acid secretion at the dorsal vagal complex.

5-CT 或 DOI 增强 TRH 类似物诱导的背侧迷走神经复合体胃酸分泌。

• DOI: 10.1152/ajpregu.1997.273.5.r1607
• 发表时间: 1997
• 期刊: The American journal of physiology
• 作者: Varanasi,S;Chi,J;StephensJr,RL
• 通讯作者: StephensJr,RL

Stimulation of the nucleus raphe obscurus produces marked serotonin release into the dorsal medulla of fed but not fasted rats--glutamatergic dependence.

刺激中缝暗核会产生显着的血清素释放到进食但未禁食的大鼠的背髓质中——谷氨酸能依赖性。

• DOI: 10.1016/0006-8993(95)00505-k
• 发表时间: 1995
• 期刊: Brain research
• 影响因子: 2.9
• 作者: Mohammed,JR;Saska,TA;Chi,J;StephensJr,RL
• 通讯作者: StephensJr,RL

Methiothepin attenuates gastric secretion and motility effects of vagal stimulants at the dorsal vagal complex.

甲硫替平减弱迷走神经刺激物对背侧迷走神经复合体的胃分泌和运动作用。

• DOI: 10.1016/s0014-2999(01)01579-5
• 发表时间: 2002
• 期刊: European journal of pharmacology
• 影响因子: 5
• 作者: Varanasi,Sridhar;Chi,Jinhan;StephensJr,RobertL
• 通讯作者: StephensJr,RobertL

Methiothepin but not methysergide antagonizes TRH effects at the dorsal vagal complex.

甲硫替平（而非美西麦角）拮抗背侧迷走神经复合体的 TRH 作用。

• DOI: 10.1111/j.1749-6632.1998.tb10231.x
• 发表时间: 1998
• 期刊: Annals of the New York Academy of Sciences
• 影响因子: 5.2
• 作者: Varanasi,S;Chi,J;Rogers,RC;StephensJr,RL
• 通讯作者: StephensJr,RL