1H NMR MEASUREMENTS OF BRAIN GABA CONCENTRATION

脑 GABA 浓度的 1H NMR 测量

• 批准号: 2270111
• 负责人: Douglas Lyle Rothman
• 金额: $ 10.23万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2270111
• 关键词: anticonvulsants; biological models; brain metabolism; diagnosis quality /standard; epilepsy; gamma aminobutyrate; human subject; laboratory rat; method development; neurochemistry; neurons; noninvasive diagnosis; nuclear magnetic resonance spectroscopy; phantom model

The GABAergic system may play a significant role in the pathogenesis as well as in the pharmacological control of epilepsy and other brain diseases. Concentrations and rates of synthesis of GABA may reflect the activity of the GABAergic system. The goal of the proposed research is to develop quantitative single volume and multivolume 1H NMR methods for measuring brain gamma amino butyric acid (GABA) concentration and rates of synthesis in human brain. The methods will be applied in studies of human subjects to determine the precision of the measurement, the accuracy of the resonance assignment, and to measure the relaxation properties needed for GABA absolute quantitation. An animal model will be used to validate the NMR method by comparison with conventional chemical assay. The development of 1H NMR methods for measuring GABA will provide an important new tool for studying the human GABAergic system.

GABA能系统可能在发病机制中起重要作用， 以及在癫痫和其他脑疾病的药理学控制中， 疾病 GABA的浓度和合成速率可能反映了 GABA能系统的活动。 拟议研究的目标是 开发定量单体积和多体积1H NMR方法， 测量脑γ-氨基丁酸（GABA）浓度和速率 大脑中的合成。 这些方法将应用于 人类受试者来确定测量的精确度， 共振分配的准确性，并测量松弛 GABA绝对定量所需的性质。 动物模型将 通过与常规方法的比较， 化学分析 GABA ~ 1H NMR测定方法的研究进展 将为研究人类GABA能提供一个重要的新工具， 系统