NUTRIENT TRANSPORT TO MOSQUITO OOCYTES

营养物质向蚊子卵母细胞的运输

• 批准号: 2021874
• 负责人: JOHN H LAW
• 金额: $ 30.97万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-02-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2021874
• 关键词: Aedes; binding proteins; density gradient ultracentrifugation; developmental nutrition; dietary iron; dietary lipid; egg /ovum; fat body; ferritin; gastrointestinal nutrient absorption; hemolymph; immunocytochemistry; insect control; lipid transport; lipoprotein lipase; northern blottings; nutrient requirement; nutrition related tag; oogenesis; receptor binding; transferrin; transferrin receptor; western blottings

All insects provide essential nutrients to their eggs. Failure to provide these nutrients will result in failure of the embryo to develop. Determination of the molecular events of transport and sequestration of nutrients in the egg should provide new targets for specific control of insect pests, especially vectors of human and animal diseases. We will investigate the transport of two essential nutrients--iron and lipids--to the oocytes of the mosquito Aedes aegypti. Since the adult female must feed on vertebrate blood in order to produce eggs, we will study the utilization of both inorganic and heme iron from the blood meal for egg production. Focus will be on the carrier proteins for transport through the hemolymph and the uptake and storage in the oocyte. This will involve iron-binding proteins, transferrin and ferritin, and receptors that allow iron to enter cells. Lipid transport from the fat body to the oocyte involves release, probably facilitated by a lipid transport particle, transport by lipoproteins, unloading at the ovary facilitated by lipoprotein lipase and a receptor for binding the lipoprotein to the cell membrane. Attempts will be made to assess the effectiveness of interfering with the function of the various macromolecular components. The ultimate goal is to provide systems that will allow development of specific materials that inhibit insect reproduction.

所有昆虫都为其卵提供必需的营养。 未能提供 这些营养物质将导致胚胎发育失败。 转运和隔离的分子事件的测定 鸡蛋中的营养物质应该为具体控制 害虫，特别是人类和动物疾病的媒介。 我们将研究两种必需营养素的运输——铁和 脂质——埃及伊蚊的卵母细胞。自从成年之后 雌性必须以脊椎动物的血液为食才能产卵，我们将 研究血粉中无机铁和血红素铁的利用 用于产蛋。 重点将放在运输的载体蛋白上 通过血淋巴以及卵母细胞的摄取和储存。 这将 涉及铁结合蛋白、转铁蛋白和铁蛋白以及受体 允许铁进入细胞。 从脂肪体到卵母细胞的脂质运输可能涉及释放 由脂质运输颗粒促进，由脂蛋白运输， 脂蛋白脂肪酶和受体促进卵巢卸载 用于将脂蛋白结合到细胞膜上。将努力 评估干扰各种功能的有效性 大分子成分。 最终目标是提供系统 将允许开发抑制昆虫的特定材料 生殖。

项目成果

Adipokinetic hormone stimulates neurones in the insect central nervous system.

脂肪运动激素刺激昆虫中枢神经系统中的神经元。

• DOI: 10.1242/jeb.198.6.1307
• 发表时间: 1995
• 期刊: The Journal of experimental biology
• 作者: Milde,JJ;Ziegler,R;Wallstein,M
• 通讯作者: Wallstein,M

Control of juvenile hormone biosynthesis. Evidence for phosphorylation of the 3-hydroxy-3-methylglutaryl coenzyme A reductase of insect corpus allatum.

• DOI: 10.1016/s0021-9258(19)68126-3
• 发表时间: 1982-02
• 期刊: The Journal of biological chemistry
• 作者: D. Monger;J. H. Law

Changes in lipoprotein composition during larval-pupal metamorphosis of an insect, Manduca sexta.

昆虫天蛾幼虫-蛹变态过程中脂蛋白组成的变化。

• 发表时间: 1986
• 期刊: The Journal of biological chemistry
• 作者: Prasad,SV;Ryan,RO;Law,JH;Wells,MA
• 通讯作者: Wells,MA

Separation of insect hemolymph proteins by cascade-mode multi-affinity chromatography.

通过级联模式多亲和层析分离昆虫血淋巴蛋白。

• DOI: 10.1002/arch.940200402
• 发表时间: 1992
• 期刊: Archives of insect biochemistry and physiology
• 影响因子: 2.2
• 作者: Samaraweera,P;Porath,J;Law,JH
• 通讯作者: Law,JH

Role of lipophorin in lipid transport to the insect egg.

脂蛋白在脂质运输至昆虫卵中的作用。

• 发表时间: 1988
• 期刊: The Journal of biological chemistry
• 作者: Kawooya,JK;Law,JH
• 通讯作者: Law,JH