Unveiling the nanoscale organisation and dynamics of synaptic vesicle pools

揭示突触小泡池的纳米级组织和动力学

• 批准号: DP190100674
• 负责人: Prof Frederic Meunier
• 金额: $ 46.91万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2019
• 资助国家: 澳大利亚
• 起止时间: 2019-01-10 至 2022-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP190100674
• 关键词: Unveiling; nanoscale; organisation; dynamics; synaptic

This project aims to uncover the role of key molecules in allowing brain cells to actively communicate with each other. Communication between neurons relies on the fusion of synaptic vesicles containing neurotransmitters with the presynaptic plasma membrane. The addition of vesicular membrane is transient as the vesicles quickly reform from the plasma membrane and refill with neurotransmitter ready for subsequent rounds of fusion. This recycling process ensures that neurons communicate efficiently, however the underpinning mechanism is unknown. This project aims to use a recently developed single synaptic vesicle super-resolution tracking method to establish how Myosin-VI and Synapsin-IIa orchestrate this recycling in central and peripheral neurons. It will explain how neurons manage to preserve their ability to communicate.

该项目旨在揭示关键分子在让脑细胞积极相互沟通方面的作用。神经元之间的通讯依赖于含有神经递质的突触小泡与突触前质膜的融合。囊泡膜的添加是短暂的，因为囊泡迅速从质膜上重新形成并重新充满神经递质，为后续几轮融合做好准备。这种循环过程确保神经元有效地沟通，但其基础机制尚不清楚。该项目旨在使用最近开发的单突触小泡超分辨率跟踪方法来确定肌球蛋白-VI 和突触蛋白-IIa 如何在中枢和外周神经元中协调这种循环。它将解释神经元如何设法保持其交流能力。