Intra and intermolecular steps underpinning vesicular priming

支持囊泡启动的分子内和分子间步骤

• 批准号: DP170100125
• 负责人: Prof Frederic Meunier
• 金额: $ 38.52万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2017
• 资助国家: 澳大利亚
• 起止时间: 2017-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP170100125
• 关键词: Intra; intermolecular; steps; underpinning; vesicular

This project aims to discover how secretory vesicles fuse with the plasma membrane, a process called priming. The fusion of secretory vesicles by exocytosis underpins neuronal communication. Despite efforts to understand vesicular fusion, how these vesicles become fusion-competent upon arrival at the plasma membrane is unknown. This project will use single molecule imaging to assess mobility changes of key priming molecules and uncover their diffusional signature during priming. It intends to build a comprehensive model of molecular interactions that make a recently docked vesicle fusion-competent. This understanding is key to unravelling how the brain works

这个项目旨在发现分泌囊泡是如何与质膜融合的，这个过程被称为启动。胞吐作用使分泌囊泡融合，是神经元通讯的基础。尽管努力了解囊泡融合，但这些囊泡如何在到达质膜后成为融合能力尚不清楚。本项目将利用单分子成像技术评估关键启动分子的迁移率变化，揭示其在启动过程中的扩散特征。它打算建立一个分子相互作用的综合模型，使最近停靠的囊泡融合能力。这种理解是解开大脑工作原理的关键