Super-resolving neurotransmitter release machinery during priming

启动过程中超分辨神经递质释放机制

• 批准号: DP220102334
• 负责人: Prof Frederic Meunier
• 金额: $ 41.33万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2022
• 资助国家: 澳大利亚
• 起止时间: 2022-01-21 至 2025-01-20
• 项目状态: 未结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP220102334
• 关键词: Super; resolving; neurotransmitter; release; machinery

Understanding how neurons communicate in the brain is one of the most challenging feats in neuroscience. The assembly of the molecular machinery involved in communication is unknown. This grant aims to understand how priming molecules Munc18 and Munc13, undergo a series of molecular steps leading to the release of neurotransmitter. Using innovative single-molecule super-resolution imaging we will uncover how Munc18 and Munc13 are spatially and temporally organised to mediate communication. By elucidating how nanoclustering of these essential proteins enables key steps, this grant will reveal how brain cells communicate. This may then provide new opportunities to optimise underlying functions such as cognition, sensory and motor processing.

了解神经元在大脑中的交流方式是神经科学中最具挑战性的壮举之一。参与通讯的分子机器的组装尚不清楚。这项资助旨在了解启动分子Munc18和Munc13是如何经历一系列分子步骤导致神经递质释放的。使用创新的单分子超分辨率成像，我们将揭示Munc18和Munc13是如何在空间和时间上组织起来的，以调解交流。通过阐明这些基本蛋白质的纳米聚集如何实现关键步骤，这项拨款将揭示脑细胞如何通信。这可能会为优化认知、感觉和运动处理等潜在功能提供新的机会。