ORGANIZATION OF THE MAMMALIAN MITOTIC SPINDLE

哺乳动物有丝分裂纺锤体的组织

• 批准号: 2459576
• 负责人: Duane A. Compton
• 金额: $ 18.86万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2459576
• 关键词: antibody; cell free system; cytoskeletal proteins; genetic library; immunoprecipitation; laboratory mouse; microinjections; mitotic spindle apparatus; nucleic acid sequence; polymerase chain reaction; protein structure function

DESCRIPTION: The objective of the experiments proposed in this application are to identify and functionally characterize the structural proteins that comprise the spindle matrix through the following specific aims: l. Using a cell-free mitotic aster assembly system that is highly enriched in the components of the mitotic spindle matrix, the investigator will prepare antibodies that specifically recognize the three major proteins of the mitotic spindle matrix. 2. Using the antibodies prepared in Specific Aim l, the investigator will use immunodepletion and microinjection to determine if each of these proteins is required for the assembly of the mitotic spindle. 3. Using the antibodies prepared in Specific Aim l, he will isolate cDNA clones encoding each of these proteins of the mitotic spindle matrix and determine their primary amino acid sequence.

描述：本申请中提出的实验目的 是鉴定和功能性表征结构蛋白， 包括主轴基体，具体目的是： L.使用高度富集的无细胞有丝分裂星形组装系统 在有丝分裂纺锤体基质的组分中，研究者将 制备特异性识别三种主要蛋白质的抗体， 有丝分裂纺锤体基质。 2. 使用在特定目标1中制备的抗体，研究者将 使用免疫耗竭和显微注射，以确定是否这些 蛋白质是有丝分裂纺锤体组装所必需的。 3. 使用在Specific Aim 1中制备的抗体，他将分离cDNA 编码有丝分裂纺锤体基质的这些蛋白质中的每一种的克隆， 确定其一级氨基酸序列。