Enzyme Electrochemical Communication

酶电化学通讯

• 批准号: DP150103345
• 负责人: Prof Paul Bernhardt
• 金额: $ 22.06万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2015
• 资助国家: 澳大利亚
• 起止时间: 2015-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP150103345
• 关键词: Enzyme; Electrochemical; Communication

The ways that redox enzymes communicate with an electrochemical electrode are poorly understood and most systems rely on small molecule mediators as electron shuttles to complete the circuit. The few examples where direct (unmediated) enzyme electrochemistry has been achieved have relied on empirical experimental approaches in electrode modification. In this project a rational approach will be taken, starting with a mediated enzyme electrochemical system which is then systematically deconstructed to produce a minimal enzyme-electrode that is stabilised by non-covalent forces and functions without a mediator. This rational approach will provide new routes to the direct enzyme electrochemistry of other enzyme systems as yet unexplored.

氧化还原酶与电化学电极的通信方式知之甚少，大多数系统依赖于小分子介体作为电子穿梭器来完成电路。直接（非介导）酶电化学已经实现的少数例子依赖于电极修饰的经验实验方法。在该项目中，将采取合理的方法，从介导的酶电化学系统开始，然后系统地解构以产生最小的酶电极，该酶电极通过非共价力稳定，并且在没有介体的情况下起作用。这种合理的方法将提供新的途径，直接酶电化学的其他酶系统尚未探索。