ELECTRON CRYSTALLOGRAPHY OF MACROMOLECULES

大分子电子晶体学

• 批准号: 2728673
• 负责人: Wah Chiu
• 金额: $ 1.5万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2728673
• 关键词: bioimaging /biomedical imaging; electron crystallography; meeting /conference /symposium; protein protein interaction; structural biology; technology /technique development; workshop

We propose to organize a Workshop on Electron Crystallography of Macromolecules at Granlibakken Conference Center in Lake Tahoe, California, between December 9-12, 1998. The motivation of this workshop is to based on the recent advances in this field of structural biology capable of resolving biological structure details at 3-10 Angstrom units resolution range. The Workshop will focus on recent dramatic advances in four branches of electron crystallography, emphasizing both methodology development and the elucidation of intermolecular interactions in large macromolecular assemblies. Particular attention will be paid to proteins - both integral membrane components and non-membrane proteins - in crystalline monolayers; probing intermolecular interactions by fitting high resolution subunit structures into lower resolution maps of intact complexes; analysis of filaments and free-standing particles at subnanometer resolution; and a novel method of phasing native X-ray data based on exploiting EM- derived molecular envelopes and non-crystallographic symmetry. The goal of the Workshop is to summarize these and related lines of investigation, assess the prospects for further methodological improvement (higher resolution, accelerated analysis), and stimulate their application to a more extensive set of biological problems. Another important purpose of this Workshop is to disseminate this emerging structural biology tool to the cell and molecular biology community. We will make an attempt to attract participants from non structural biology discipline attending this meeting via a variety of means of advertising the Workshop and scheduling it right before the 1998 Cell Biology Annual Conference to be held in San Francisco. For a broader dissemination, we plan to publish a special issue of the envisaged topics in the Journal of Structural Biology contributed from the invited participants.

我们建议组织一次电子晶体学研讨会， 大分子在太浩湖的Granlibakken会议中心， 加州，1998年12月9日至12日。 这样做的动机 研讨会将根据这一领域的最新进展， 生物学能够在3-10分辨生物结构细节 埃单位分辨率范围。 研讨会将重点放在最近的 在电子晶体学的四个分支中取得了巨大的进步， 强调方法的发展和阐明 大分子组装体中的分子间相互作用。 将特别注意蛋白质-既包括完整的膜 组分和非膜蛋白-在结晶单层中; 通过拟合高分辨率亚基探测分子间相互作用 完整复合物的低分辨率图谱; 亚纳米分辨率的细丝和独立颗粒;以及 一种新的方法，定相天然X射线数据的基础上，利用EM- 衍生的分子包络和非晶体学对称性。目标 研讨会的目的是总结这些和相关的路线， 调查，评估进一步方法的前景， 改进（更高的分辨率，加速分析），并刺激 将其应用于更广泛的生物学问题。 本次研讨会的另一个重要目的是传播这一信息， 新兴的结构生物学工具， 社区 我们将努力吸引来自非 结构生物学学科通过各种方式参加这次会议， 宣传讲习班的方式，并在讲习班开始前安排讲习班。 1998年细胞生物学年会将在旧金山弗朗西斯科举行。 为 为了更广泛地传播，我们计划出版一期《 《结构生物学杂志》中设想的主题， 被邀请的参与者。