Stanford-SLAC CryoET Specimen Preparation Service Center (SCSC)
斯坦福-SLAC CryoET 标本制备服务中心 (SCSC)
基本信息
- 批准号:10818212
- 负责人:
- 金额:$ 330.19万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-09-16 至 2024-08-31
- 项目状态:已结题
- 来源:
- 关键词:AcademiaAdoptedAdvisory CommitteesAreaAwardBiocompatible MaterialsBiologicalCellsCollaborationsCommunicationCommunitiesComplexCryo-electron tomographyCryoelectron MicroscopyData CollectionDedicationsEducational CurriculumEducational workshopElectron MicroscopeEquipmentFluorescence MicroscopyFreezingFundingGoalsHydration statusIceIn SituIndustryInfrastructureInstitutionIonsLaboratoriesLiftingMacromolecular ComplexesMethodsMicroscopeOrganellesPreparationProcessProfessional OrganizationsProtocols documentationRecurrenceRequest for ApplicationsResearchResource AllocationResourcesSamplingScanningScanning Electron MicroscopyServicesSpecimenSubcellular structureTechniquesTechnologyThickThinnessTissue SampleTissuesTitanTrainingTraining ActivityUnited States National Institutes of HealthWorkcryogenicselectron tomographyexperimental studyimaging modalityinnovative technologiesnanometer resolutionresponsestructural biologysymposiumtoolweb portal
项目摘要
Project Summary
In response to the NIH RFA-RM-19-009, we propose to establish the Stanford-SLAC
CryoET Specimen Preparation Service Center (SCSC) to accomplish four specific aims: (1)
establish a platform, which is available to the scientific community at large, to streamline the
preparation of samples suitable for downstream cryogenic electron tomography (cryoET) data
collection; (2) provide access to advanced cryo-specimen preparation techniques for a wide
range of samples: macromolecular complexes, microcrystals of biological materials, cell
lysates, organelles, cells and tissues; (3) generate a training curriculum for new users on the
preparation of frozen, hydrated biological specimens, with the option of carrying out correlative
cryo-fluorescence light microscopy (cryoFLM), cryo-focused ion beam scanning electron
microscopy (cryoFIB), and cryoET; and (4) adopt new methods for sample preparation using
innovative technologies developed elsewhere. In this proposal, we will leverage our existing
facilities and in-house expertise in each of the following imaging modalities and their integration
into a correlated sample preparation workflow: cryoFLM, cryoFIB, and cryoET. We will establish
all the necessary equipment dedicated to the proposed service and training activities. We will
prepare video curriculum materials to train new users in the aforementioned protocols. We will
adapt our existing infrastructure to manage project administration and resource allocations.
Administrative support is in place to help users with lodging arrangements, as well as laboratory
on-boarding and specimen biosafety approvals. We have a well set- up communication
infrastructure for remote users to participate in actual experiments, particularly for cryoFLM,
cryoFIB, and cryoET. We will provide access to existing Titan Krios microscopes to evaluate
whether the prepared samples are ready for data collection in the associated Hub, which will
be set up at another institution with separate NIH support. We will make our existing Stanford-
SLAC cryo-specimen preparation equipment available on a limited basis immediately after this
proposal is funded. We anticipate serving ~40 users per year once the Center is fully
operational in early Year 2 of the award period. We will also offer regular hands- on workshops
to train ~10-12 new users per year. We will disseminate our resources to the broad community
via a web portal and booths at professional societies’ annual conferences. The number of users
will increase in subsequent years as we and our recurring users optimize the use of the Center’s
resources. In Years 3-6, we will work closely with collaborators pioneering advanced protocols
such as semi-automated and automated cryoFIB and “lift-out” for tissue samples to implement
them in the proposed Center. Lastly, we will assemble a Scientific Advisory Committee of
experts to guide the practices of our Service Center and the ongoing implementation of cutting-
edge technologies.
项目摘要
为了响应NIH RFA-RM-19-009,我们建议建立斯坦福-SLAC
CryoET标本制备服务中心(SCSC)将实现四个具体目标:(1)
建立一个供广大科学界使用的平台,
适合于下游低温电子断层摄影(cryoET)数据的样品的制备
收集;(2)提供先进的冷冻标本制备技术,
样品范围:高分子复合物,生物材料的微晶,细胞
裂解物,细胞器,细胞和组织;(3)为新用户提供关于
冷冻、水合生物标本的制备,可选择进行相关的
低温聚焦离子束扫描电子显微镜
(4)采用新的样品制备方法,
其他地方开发的创新技术。在本提案中,我们将利用现有的
以下每种成像模式及其集成的设施和内部专业知识
相关的样品制备工作流程:cryoFLM、cryoFIB和cryoET。我们将建立
专用于拟议服务和培训活动的所有必要设备。我们将
制作录像教材,对新用户进行上述议定书方面的培训。我们将
调整我们现有的基础设施,以管理项目管理和资源分配。
行政支持到位,以帮助用户安排住宿,以及实验室
入职和样本生物安全批准。我们有一个良好的沟通机制
远程用户参与实际实验的基础设施,特别是cryoFLM,
cryoFIB和cryoET。我们将提供现有的Titan Krios显微镜,
准备好的样品是否已准备好在相关中心进行数据收集,
在另一个机构设立,并得到NIH的单独支持。我们将把现有的斯坦福大学-
在此之后立即提供有限的SLAC冷冻标本制备设备
提案得到资助。我们预计,一旦中心完全建成,每年将为约40名用户提供服务。
在奖励期的第二年开始运作。我们还将定期举办实践研讨会
每年培训约10-12名新用户。我们将把我们的资源传播给广大社会
通过门户网站和专业协会年度会议上的展位。用户数量
在接下来的几年里,随着我们和我们的经常性用户优化中心的使用,
资源在第3-6年,我们将与开拓先进协议的合作者密切合作
例如半自动化和自动化的冷冻FIB和用于组织样本的“提出”,
在拟议中的中心。最后,我们将组建一个科学咨询委员会,
专家指导我们的服务中心的做法和正在进行的实施切割-
边缘技术。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Wah Chiu其他文献
Wah Chiu的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Wah Chiu', 18)}}的其他基金
Cryo-ET Structural Biology of Herpesvirus Infection and Morphogenesis In Situ.
疱疹病毒感染和原位形态发生的 Cryo-ET 结构生物学。
- 批准号:
10192472 - 财政年份:2021
- 资助金额:
$ 330.19万 - 项目类别:
Cryo-ET Structural Biology of Herpesvirus Infection and Morphogenesis In Situ.
疱疹病毒感染和原位形态发生的 Cryo-ET 结构生物学。
- 批准号:
10352451 - 财政年份:2021
- 资助金额:
$ 330.19万 - 项目类别:
Stanford-SLAC CryoET Specimen Preparation Service Center (SCSC)
斯坦福-SLAC CryoET 标本制备服务中心 (SCSC)
- 批准号:
10588756 - 财政年份:2020
- 资助金额:
$ 330.19万 - 项目类别:
Stanford-SLAC CryoET Specimen Preparation Service Center (SCSC) Supplement
斯坦福-SLAC CryoET 标本制备服务中心 (SCSC) 补充资料
- 批准号:
10265915 - 财政年份:2020
- 资助金额:
$ 330.19万 - 项目类别:
Stanford-SLAC CryoET Specimen Preparation Service Center (SCSC)
斯坦福-SLAC CryoET 标本制备服务中心 (SCSC)
- 批准号:
10264893 - 财政年份:2020
- 资助金额:
$ 330.19万 - 项目类别:
Stanford-SLAC CryoET Specimen Preparation Service Center (SCSC)
斯坦福-SLAC CryoET 标本制备服务中心 (SCSC)
- 批准号:
10054621 - 财政年份:2020
- 资助金额:
$ 330.19万 - 项目类别:
Stanford-SLAC CryoET Specimen Preparation Service Center (SCSC)
斯坦福-SLAC CryoET 标本制备服务中心 (SCSC)
- 批准号:
10473758 - 财政年份:2020
- 资助金额:
$ 330.19万 - 项目类别:
相似海外基金
How novices write code: discovering best practices and how they can be adopted
新手如何编写代码:发现最佳实践以及如何采用它们
- 批准号:
2315783 - 财政年份:2023
- 资助金额:
$ 330.19万 - 项目类别:
Standard Grant
One or Several Mothers: The Adopted Child as Critical and Clinical Subject
一位或多位母亲:收养的孩子作为关键和临床对象
- 批准号:
2719534 - 财政年份:2022
- 资助金额:
$ 330.19万 - 项目类别:
Studentship
A material investigation of the ceramic shards excavated from the Omuro Ninsei kiln site: Production techniques adopted by Nonomura Ninsei.
对大室仁清窑遗址出土的陶瓷碎片进行材质调查:野野村仁清采用的生产技术。
- 批准号:
20K01113 - 财政年份:2020
- 资助金额:
$ 330.19万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
A comparative study of disabled children and their adopted maternal figures in French and English Romantic Literature
英法浪漫主义文学中残疾儿童及其收养母亲形象的比较研究
- 批准号:
2633211 - 财政年份:2020
- 资助金额:
$ 330.19万 - 项目类别:
Studentship
A comparative study of disabled children and their adopted maternal figures in French and English Romantic Literature
英法浪漫主义文学中残疾儿童及其收养母亲形象的比较研究
- 批准号:
2436895 - 财政年份:2020
- 资助金额:
$ 330.19万 - 项目类别:
Studentship
A comparative study of disabled children and their adopted maternal figures in French and English Romantic Literature
英法浪漫主义文学中残疾儿童及其收养母亲形象的比较研究
- 批准号:
2633207 - 财政年份:2020
- 资助金额:
$ 330.19万 - 项目类别:
Studentship
A Study on Mutual Funds Adopted for Individual Defined Contribution Pension Plans
个人设定缴存养老金计划采用共同基金的研究
- 批准号:
19K01745 - 财政年份:2019
- 资助金额:
$ 330.19万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The limits of development: State structural policy, comparing systems adopted in two European mountain regions (1945-1989)
发展的限制:国家结构政策,比较欧洲两个山区采用的制度(1945-1989)
- 批准号:
426559561 - 财政年份:2019
- 资助金额:
$ 330.19万 - 项目类别:
Research Grants
Securing a Sense of Safety for Adopted Children in Middle Childhood
确保被收养儿童的中期安全感
- 批准号:
2236701 - 财政年份:2019
- 资助金额:
$ 330.19万 - 项目类别:
Studentship
Structural and functional analyses of a bacterial protein translocation domain that has adopted diverse pathogenic effector functions within host cells
对宿主细胞内采用多种致病效应功能的细菌蛋白易位结构域进行结构和功能分析
- 批准号:
415543446 - 财政年份:2019
- 资助金额:
$ 330.19万 - 项目类别:
Research Fellowships