Stanford-SLAC CryoET Specimen Preparation Service Center (SCSC) Supplement
斯坦福-SLAC CryoET 标本制备服务中心 (SCSC) 补充资料
基本信息
- 批准号:10265915
- 负责人:
- 金额:$ 37.73万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-09-16 至 2024-08-31
- 项目状态:已结题
- 来源:
- 关键词:AcademiaAdvisory CommitteesAreaAwardBiocompatible MaterialsBiomedical ResearchBudgetsCellsCellular StructuresCollaborationsCommunicationCommunitiesComplexConsultCryo-electron tomographyCryoelectron MicroscopyData CollectionEducational CurriculumEducational workshopElectron MicroscopeEquipmentEuropeanEvaluationFluorescence MicroscopyFreezingFundingGoalsGrantHourHydration statusIn SituIndustryInfrastructureInstitutionInvestigationIonsLaboratoriesLiftingMacromolecular ComplexesManufacturer NameMethodsMolecular StructureOrganellesPerformancePreparationProceduresProcessProtocols documentationRequest for ApplicationsResearchResearch PersonnelResolutionResource AllocationSamplingScanningScanning Electron MicroscopyScheduleScientistSelection CriteriaServicesSliceSpecimenSubcellular structureTechniquesTechnologyThickTissue SampleTissuesTitanTrainingTraining ActivityWorkdata managementexperienceexperimental studyimaging modalityinstrumentinstrumentationinterestmemberoperationpressureprogramsprototyperesponsestructural biologytool
项目摘要
Project Summary
Upgrade of Aquilos to Aquilos 2 cryo-focused ion beam scanning electron microscope. In our original
proposed budget, we requested ~$1M for a focused ion beam instrument. We did not specify the
manufacturer of such instrument (i.e. Thermo Fisher or Zeiss). The recent announcement from Thermo
Fisher of the Aquilos 2 (~$1.38M), offers multiple exciting features. It has the capabilities of performing
lift-out, slice and view and automated milling with 20+ lamellae in 13 hours of operation. These results
suggest that cryoFIB can become an automated procedure on very thick, high-pressure vitrified samples.
This would be a highly sought-after capability of our Service Center, because we anticipate that there
will be a significant demand if we can begin to work with tissues tens to hundreds of microns in
thickness. These performance specifications and the results shown by Thermo Fisher are exciting. If
funded, we will also investigate whether Zeiss can offer a similar or superior instrument before we make
a purchase decision. Certainly, we will consult with our European colleagues who have the prototypes of
the new instrument in terms of its robustness as a user facility as in our Center.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Wah Chiu其他文献
Wah Chiu的其他文献
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{{ truncateString('Wah Chiu', 18)}}的其他基金
Cryo-ET Structural Biology of Herpesvirus Infection and Morphogenesis In Situ.
疱疹病毒感染和原位形态发生的 Cryo-ET 结构生物学。
- 批准号:
10192472 - 财政年份:2021
- 资助金额:
$ 37.73万 - 项目类别:
Cryo-ET Structural Biology of Herpesvirus Infection and Morphogenesis In Situ.
疱疹病毒感染和原位形态发生的 Cryo-ET 结构生物学。
- 批准号:
10352451 - 财政年份:2021
- 资助金额:
$ 37.73万 - 项目类别:
Stanford-SLAC CryoET Specimen Preparation Service Center (SCSC)
斯坦福-SLAC CryoET 标本制备服务中心 (SCSC)
- 批准号:
10818212 - 财政年份:2020
- 资助金额:
$ 37.73万 - 项目类别:
Stanford-SLAC CryoET Specimen Preparation Service Center (SCSC)
斯坦福-SLAC CryoET 标本制备服务中心 (SCSC)
- 批准号:
10588756 - 财政年份:2020
- 资助金额:
$ 37.73万 - 项目类别:
Stanford-SLAC CryoET Specimen Preparation Service Center (SCSC)
斯坦福-SLAC CryoET 标本制备服务中心 (SCSC)
- 批准号:
10264893 - 财政年份:2020
- 资助金额:
$ 37.73万 - 项目类别:
Stanford-SLAC CryoET Specimen Preparation Service Center (SCSC)
斯坦福-SLAC CryoET 标本制备服务中心 (SCSC)
- 批准号:
10473758 - 财政年份:2020
- 资助金额:
$ 37.73万 - 项目类别:
Stanford-SLAC CryoET Specimen Preparation Service Center (SCSC)
斯坦福-SLAC CryoET 标本制备服务中心 (SCSC)
- 批准号:
10054621 - 财政年份:2020
- 资助金额:
$ 37.73万 - 项目类别:
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