Stanford-SLAC CryoET Specimen Preparation Service Center (SCSC) Supplement

斯坦福-SLAC CryoET 标本制备服务中心 (SCSC) 补充资料

• 批准号: 10265915
• 负责人: Wah Chiu
• 金额: $ 37.73万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-16 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10265915
• 关键词: Academia; Advisory Committees; Area; Award; Biocompatible Materials; Biomedical Research; Budgets; Cells; Cellular Structures; Collaborations; Communication; Communities; Complex; Consult; Cryo-electron tomography; Cryoelectron Microscopy; Data Collection; Educational Curriculum; Educational workshop; Electron Microscope; Equipment; European; Evaluation; Fluorescence Microscopy; Freezing; Funding; Goals; Grant; Hour; Hydration status; In Situ; Industry; Infrastructure; Institution; Investigation; Ions; Laboratories; Lifting; Macromolecular Complexes; Manufacturer Name; Methods; Molecular Structure; Organelles; Performance; Preparation; Procedures; Process; Protocols documentation; Request for Applications; Research; Research Personnel; Resolution; Resource Allocation; Sampling; Scanning; Scanning Electron Microscopy; Schedule; Scientist; Selection Criteria; Services; Slice; Specimen; Subcellular structure; Techniques; Technology; Thick; Tissue Sample; Tissues; Titan; Training; Training Activity; Work; data management; experience; experimental study; imaging modality; instrument; instrumentation; interest; member; operation; pressure; programs; prototype; response; structural biology; tool

Project Summary Upgrade of Aquilos to Aquilos 2 cryo-focused ion beam scanning electron microscope. In our original proposed budget, we requested ~$1M for a focused ion beam instrument. We did not specify the manufacturer of such instrument (i.e. Thermo Fisher or Zeiss). The recent announcement from Thermo Fisher of the Aquilos 2 (~$1.38M), offers multiple exciting features. It has the capabilities of performing lift-out, slice and view and automated milling with 20+ lamellae in 13 hours of operation. These results suggest that cryoFIB can become an automated procedure on very thick, high-pressure vitrified samples. This would be a highly sought-after capability of our Service Center, because we anticipate that there will be a significant demand if we can begin to work with tissues tens to hundreds of microns in thickness. These performance specifications and the results shown by Thermo Fisher are exciting. If funded, we will also investigate whether Zeiss can offer a similar or superior instrument before we make a purchase decision. Certainly, we will consult with our European colleagues who have the prototypes of the new instrument in terms of its robustness as a user facility as in our Center.

项目摘要 Aquilos升级为Aquilos 2低温聚焦离子束扫描电子显微镜。在我们的原版中 在拟议的预算中，我们为一台聚焦离子束仪器申请了约100万美元。我们没有具体说明 此类仪器的制造商(即Thermo Fisher或Zeiss)。来自Thermo的最新声明 Aquilos 2的费舍尔(约138万美元)提供了多种令人兴奋的功能。它有表演的能力 提拉、切片和查看，并在13小时内使用20多片自动铣削。这些结果 这表明，对于非常厚的高压玻璃化样品，冷冻FIB可以成为一种自动化的程序。 这将是我们服务中心非常抢手的功能，因为我们预计在那里 如果我们能开始处理几十到几百微米的组织，这将是一个巨大的需求 厚度。这些性能指标和Thermo Fisher展示的结果令人兴奋。如果 ，我们还将调查蔡司是否可以在我们制造之前提供类似或更好的仪器 一个购买决定。当然，我们将与我们的欧洲同事进行磋商，他们拥有 新仪器作为用户设施的健壮性，就像我们中心一样。