The Stanford-SLAC CryoEM Center

斯坦福大学-SLAC 冷冻电镜中心

基本信息

批准号：
10621208
负责人：
Wah Chiu
金额：
$ 490.13万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-05-01 至 2024-09-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10621208
关键词：
Administrator Adopted Advisory Committees Archives Books Collaborations Computer software Computers Cryo-electron tomography Cryoelectron Microscopy Data Data Collection Department of Energy Deposition Development Educational Curriculum Educational workshop Electron Microscope Electron Microscopy Electronics Electrons Environment Evaluation Feedback Generations Geographic Locations Geography Health Home Human Image Infrastructure Institution International Laboratories Learning Light Maps Metadata Methods Modeling Online Systems Persons Physics Preparation Process Professional Organizations Protocols documentation Readiness Request for Applications Research Research Personnel Research Project Grants Resolution Resources Roentgen Rays Running Sampling Scientist Source Specialist Specimen Structural Biologist Structure Synchrotrons System Techniques Technology Three-Dimensional Image Time Training Training Programs Universities Validation atomic data computer cluster computer infrastructure computing resources contrast imaging data acquisition data exchange data quality data repository data streams detector electron diffraction experience follow-up free-electron laser image archival system image processing innovation instrument meetings next generation operation peer preservation programs prospective protein data bank recruit residence response skills structural biology technological innovation theories tool

项目摘要

Summary Stanford University and the SLAC National Accelerator Laboratory (SLAC) propose to host a National Center for Cryoelectron Microscopy – The Stanford-SLAC CryoEM Center (S2C2) – to meet the emerging national need for cryoEM as a tool for atomic-resolution structural biology. The proposed S2C2 will (1) establish, and keep at the forefront, state-of-the-art cryo-electron microscopes to satisfy users’ needs for atomic-resolution image data, (2) archive image data with appropriate metadata, (3) provide computing resources to assess data quality in real time, (4) inform and recruit potential users across the U.S. about the public accessibility of the Center, (5) establish an open, fair, transparent and efficient process to select user proposals based on scientific impact and specimen readiness, regardless of users’ geographic locations or affiliations, (6) help users to overcome technical hurdles and enable them to obtain high-resolution cryoEM structures quickly with a rigorous validation protocol, (7) form and facilitate a user network to exchange information seamlessly, (8) train users to become independent cryoEM investigators, (9) integrate user and trainee feedback into a continuous loop of facility enhancement, and (10) optimize operations to achieve the above tasks effectively and efficiently. Dr. Wah Chiu, the Contact PI, has decades of experience developing high-resolution cryoEM technologies and directing research and training enterprises across multiple institutions. Drs. Michael Schmid, Georgios Skiniotis , and Britt Hedman, also PIs of the Center, bring complementary expertise in cryoEM, structural biology, and management and operation of large-scale national user facilities. A Center Director of Operations, three skilled cryoEM specialists, a technical assistant, and a user facility administrator will carry out the S2C2’s day-to-day operations, including data collection, quality assessment, and cross-training. This S2C2 will reside in a new building at SLAC. It will leverage SLAC’s expertise in serving thousands of users each year at its renowned X-ray synchrotron and free electron laser user facilities. An Advisory Committee comprised of external expert scientists as well as user representatives will provide advice regarding S2C2 operations and development. Our Center will build on the unique strengths and support from Stanford and SLAC, and will create a unique environment that combines high throughput cryoEM data generation with training the next generation of cryoEM scientists.

概括斯坦福大学和SLAC国家加速器实验室（SLAC）提议主持国家中心对于Cyoelectron显微镜 - Stanford-SLAC冷冻中心（S2C2） - 与新兴国家见面需要冷冻作为原子分辨率结构生物学的工具。拟议的S2C2将（1）机构，并保持最前沿，最先进的冷冻电子显微镜，以满足用户对原子分辨率的需求图像数据，（2）具有适当元数据的存档图像数据，（3）为评估数据提供计算资源实时质量，（4）向美国各地的潜在用户告知并招募潜在用户中心，（5）建立一个开放，公平，透明和高效的过程，以根据科学影响和标本准备就绪，无论用户的地理位置或分支机构如何，（6）帮助用户克服技术障碍，并使他们能够快速获得高分辨率的冷冻结构严格的验证协议，（7）形式并促进用户网络无缝交换信息，（8）培训用户成为独立冷冻调查人员，（9）综合用户和受训者的反馈设施增强的连续循环，以及（10）优化操作以有效地实现上述任务并有效。联系PI的Wah Chiu博士拥有数十年来开发高分辨率冷冻的经验技术和指导跨多个机构的研究和培训企业。博士。迈克尔·施密德（Michael Schmid）， Georgios Skiniotis和中心的PIS Britt Hedman带来了Cryoem的完成者专业知识，大规模国家用户设施的结构生物学以及管理和运营。中心主任操作，三名熟练的冷冻专家，一名技术助理和用户设施管理员将携带消除S2C2的日常操作，包括数据收集，质量评估和交叉培训。这 S2C2将居住在SLAC的新建筑物中。它将利用SLAC的专业知识为数千个用户服务一年在其著名的X射线同步器和免费的电子激光用户设施。咨询委员会完成外部专家以及用户代表将提供重要的建议S2C2 运营与发展。我们的中心将基于斯坦福大学的独特优势和支持 SLAC，并将创建一个独特的环境，将高吞吐量冷冻数据生成与培训下一代冷冻科学家。