The Stanford-SLAC CryoEM Center (S2C2)

项目摘要 这是为社区提供冷冻样本筛查服务的补充请求。我们将 利用我们中心现有的设备来提供这项活动。我们预计北极塔罗斯 (200 100千伏）和Tundra（100千伏）电子显微镜。我们将 招聘一名全职工作人员参加这项活动，并将通过3DEM和我们的 用户电子邮件列表。我们预计这项服务将扩大冷冻EM技术的传播， 调查人员在其机构中没有专门知识或设施。所有用户都需要应用此 通过我们现有的项目申请流程提供服务，并通过我们的用户项目评估进行评估 在为已批准的项目分配人员和仪器时间之前，委员会应进行审查。

Project Summary This is a supplement request for providing a cryo-specimen screening service to the community. We will use our existing equipment in the Center to provide this activity. We anticipate that the Talos Arctica (200 kV) and the Tundra (100 kV) electron microscopes will be used for the proposed activity. We will recruit a full-time staff member for this activity and will advertise our service through 3DEM and our user email lists. We anticipate that this service will expand the dissemination of cryoEM technology to investigators who do not have expertise or facilities in their institutions. All the users need to apply this service via our existing project application process and evaluated by our user project evaluation committee prior to allocating staff and instrument time for the approved projects.