The Stanford-SLAC CryoEM Center

项目摘要 斯坦福大学和SLAC国家加速器实验室（SLAC）提议举办一个 国家低温电子显微镜中心-斯坦福-SLAC低温电子显微镜中心（S2 C2） - 为了满足国家对cryoEM作为原子分辨率结构分析工具的需求， 生物学拟议的S2 C2将（1）建立并保持在最前沿，最先进的低温， 电子显微镜，以满足用户对原子分辨率图像数据的需求，（2）存档图像 数据与适当的元数据，（3）提供计算资源，以评估数据质量在真实的 时间，（4）通知和招募美国各地的潜在用户关于公共可访问性的信息。 中心，（5）建立公开、公正、透明、高效的用户提案遴选流程 基于科学影响和标本准备，无论用户的地理位置或 （6）帮助用户克服技术障碍，使他们能够获得高质量的产品， 分辨率cryoEM结构快速与严格的验证协议，（7）形成和促进 用户网络无缝交换信息，（8）培养用户独立自主 cryoEM研究者，（9）将用户和受训者的反馈整合到设施的连续循环中 （10）优化运营，有效实现上述任务， 有效地联系PI Wah Chiu博士拥有数十年开发高分辨率 cryoEM技术和指导跨多个机构的研究和培训企业。 Drs. Michael Schmid和布里特海德曼也是该中心的PI，他们带来了互补的专业知识 在cryoEM、结构生物学以及大规模国家用户管理和操作方面 设施一名中心运营总监，三名熟练的cryoEM专家，一名技术助理， 用户设施管理员将执行S2 C2的日常操作，包括数据 收集、质量评估和交叉培训。该S2 C2将位于 SLAC。它将利用SLAC的专业知识，每年在其著名的 X射线同步加速器和自由电子激光用户设施。一个咨询委员会， 外部专家科学家以及用户代表将提供有关S2 C2的建议 业务和发展。我们的中心将建立在独特的优势和支持， 斯坦福大学和SLAC，并将创建一个独特的环境，结合高吞吐量 cryoEM数据生成与培训下一代cryoEM科学家。

Project Summary Stanford University and the SLAC National Accelerator Laboratory (SLAC) propose to host a National Center for Cryogenic electron Microscopy – The Stanford-SLAC CryoEM Center (S2C2) – to meet the emerging national need for cryoEM as a tool for atomic-resolution structural biology. The proposed S2C2 will (1) establish and keep at the forefront, state-of-the-art cryo- electron microscopes to satisfy users’ needs for atomic-resolution image data, (2) archive image data with appropriate metadata, (3) provide computing resources to assess data quality in real time, (4) inform and recruit potential users across the U.S. about the public accessibility of the Center, (5) establish an open, fair, transparent and efficient process to select user proposals based on scientific impact and specimen readiness, regardless of users’ geographic locations or affiliations, (6) help users to overcome technical hurdles and enable them to obtain high- resolution cryoEM structures quickly with a rigorous validation protocol, (7) form and facilitate a user network to exchange information seamlessly, (8) train users to become independent cryoEM investigators, (9) integrate user and trainee feedback into a continuous loop of facility enhancement, and (10) optimize operations to achieve the above tasks effectively and efficiently. Dr. Wah Chiu, the Contact PI, has decades of experience developing high-resolution cryoEM technologies and directing research and training enterprises across multiple institutions. Drs. Michael Schmid and Britt Hedman, also PIs of the Center, bring complementary expertise in cryoEM, structural biology, and management and operation of large-scale national user facilities. A Center Director of Operations, three skilled cryoEM specialists, a technical assistant, and a user facility administrator will carry out the S2C2’s day-to-day operations, including data collection, quality assessment, and cross-training. This S2C2 will reside in a new building at SLAC. It will leverage SLAC’s expertise in serving thousands of users each year at its renowned X-ray synchrotron and free electron laser user facilities. An Advisory Committee composed of external expert scientists as well as user representatives will provide advice regarding S2C2 operations and development. Our Center will build on the unique strengths and support from Stanford and SLAC, and will create a unique environment that combines high throughput cryoEM data generation with training the next generation of cryoEM scientists.