The Stanford-SLAC CryoEM Center

斯坦福大学-SLAC 冷冻电镜中心

• 批准号: 10615525
• 负责人: Wah Chiu
• 金额: $ 37.84万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10615525
• 关键词: Administrator; Adopted; Advisory Committees; Archives; Collaborations; Computer software; Computers; Cryo-electron tomography; Cryoelectron Microscopy; Data; Data Collection; Department of Energy; Deposition; Development; Educational Curriculum; Educational workshop; Electron Microscope; Electron Microscopy; Electrons; Environment; Evaluation; Feedback; Generations; Geographic Locations; Geography; Health; Home; Human; Image; Infrastructure; Institution; International; Laboratories; Learning; Light; Maps; Metadata; Methods; Modeling; Online Systems; Persons; Physics; Preparation; Process; Professional Organizations; Protocols documentation; Readiness; Request for Applications; Research; Research Personnel; Research Project Grants; Research Training; Resolution; Resources; Roentgen Rays; Running; Sampling; Scientist; Ships; Source; Specialist; Specimen; Structural Biologist; Structure; Synchrotrons; System; Techniques; Technology; Three-Dimensional Image; Time; Training; Training Programs; Universities; Validation; atomic data; base; computer cluster; computer infrastructure; computing resources; contrast imaging; cryogenics; data acquisition; data exchange; data quality; data repository; data streams; detector; electron diffraction; experience; follow-up; free-electron laser; image archival system; image processing; innovation; instrument; meetings; next generation; operation; peer; preservation; programs; prospective; protein data bank; recruit; residence; response; structural biology; theories; tool

Project Summary Stanford University and the SLAC National Accelerator Laboratory (SLAC) propose to host a National Center for Cryogenic electron Microscopy – The Stanford-SLAC CryoEM Center (S2C2) – to meet the emerging national need for cryoEM as a tool for atomic-resolution structural biology. The proposed S2C2 will (1) establish and keep at the forefront, state-of-the-art cryo- electron microscopes to satisfy users’ needs for atomic-resolution image data, (2) archive image data with appropriate metadata, (3) provide computing resources to assess data quality in real time, (4) inform and recruit potential users across the U.S. about the public accessibility of the Center, (5) establish an open, fair, transparent and efficient process to select user proposals based on scientific impact and specimen readiness, regardless of users’ geographic locations or affiliations, (6) help users to overcome technical hurdles and enable them to obtain high- resolution cryoEM structures quickly with a rigorous validation protocol, (7) form and facilitate a user network to exchange information seamlessly, (8) train users to become independent cryoEM investigators, (9) integrate user and trainee feedback into a continuous loop of facility enhancement, and (10) optimize operations to achieve the above tasks effectively and efficiently. Dr. Wah Chiu, the Contact PI, has decades of experience developing high-resolution cryoEM technologies and directing research and training enterprises across multiple institutions. Drs. Michael Schmid and Britt Hedman, also PIs of the Center, bring complementary expertise in cryoEM, structural biology, and management and operation of large-scale national user facilities. A Center Director of Operations, three skilled cryoEM specialists, a technical assistant, and a user facility administrator will carry out the S2C2’s day-to-day operations, including data collection, quality assessment, and cross-training. This S2C2 will reside in a new building at SLAC. It will leverage SLAC’s expertise in serving thousands of users each year at its renowned X-ray synchrotron and free electron laser user facilities. An Advisory Committee composed of external expert scientists as well as user representatives will provide advice regarding S2C2 operations and development. Our Center will build on the unique strengths and support from Stanford and SLAC, and will create a unique environment that combines high throughput cryoEM data generation with training the next generation of cryoEM scientists.

项目概要 斯坦福大学和 SLAC 国家加速器实验室 (SLAC) 提议主办 国家低温电子显微镜中心 – 斯坦福-SLAC 冷冻电镜中心 (S2C2) – 满足国家对冷冻电镜作为原子分辨率结构工具的新兴需求 生物学。拟议的 S2C2 将 (1) 建立并保持最先进的冷冻技术 电子显微镜以满足用户对原子分辨率图像数据的需求，（2）存档图像 具有适当元数据的数据，（3）提供计算资源来实时评估数据质量 (4) 向全美各地的潜在用户通报并招募有关该网站的公众可访问性的信息 中心，（5）建立公开、公平、透明、高效的用户提案评选流程 基于科学影响和样本准备情况，无论用户的地理位置或 隶属关系，（6）帮助用户克服技术障碍，使他们获得高 通过严格的验证协议快速解析冷冻电镜结构，(7) 形成并促进 用户网络无缝交换信息，（8）培养用户独立 冷冻电镜研究人员，(9) 将用户和学员的反馈整合到设施的连续循环中 （十）优化运营，有效实现上述任务 高效。 Wah Chiu 博士是联系人 PI，拥有数十年开发高分辨率的经验 冷冻电镜技术并指导多个机构的研究和培训企业。 博士。 Michael Schmid 和 Britt Hedman 也是该中心的 PI，他们带来了互补的专业知识 冷冻电镜、结构生物学、全国大型用户管理运营 设施。一名中心运营总监、三名熟练的冷冻电镜专家、一名技术助理、 用户设施管理员将执行S2C2的日常操作，包括数据 收集、质量评估和交叉培训。该 S2C2 将驻留在位于 SLAC。它将利用 SLAC 的专业知识，每年为数以千计的用户提供服务 X射线同步加速器和自由电子激光用户设施。咨询委员会由以下人员组成 外部专家科学家以及用户代表将提供有关S2C2的建议 运营和发展。我们的中心将凭借独特的优势和来自以下机构的支持 斯坦福大学和 SLAC，将创建一个结合高吞吐量的独特环境 生成冷冻电镜数据并培训下一代冷冻电镜科学家。