SPECIALIZED CENTER OF RESEARCH IN ARTERIOSCLEROSIS

本申请的总体主题是异常甘油三酯的作用 和脂肪酸代谢在动脉粥样硬化病因学中的作用。 0006项目 （W.和J. Patsch）是餐后脂蛋白， 目标识别观察到的相关性的分子基础 餐后脂血症持续时间与冠心病发生率之间 动脉疾病 项目0010（J.B. Massey）研究了脂质的作用 转运蛋白和脂质转运蛋白对脂蛋白代谢的影响， in vivo. 项目0011（H. J. Pownall）侧重于以下机制和作用： 一种刺激细胞内脂肪酸形成的血浆肽 酯盐酸盐的褪 项目0010-0011均使用仓鼠作为动物模型， 饮食引起的动脉粥样硬化 在项目0012（J.D. Morrisett），合成 将研究Lp[a]的催化剂，特别强调 餐后脂血症对富含脂蛋白的Lp[a]形成的影响 颗粒及其在体内的脂解作用。 项目0013：D. P.通过）是 旨在确定特定的脂蛋白及其成分 调节细胞特异性细胞因子的产生和分泌。 的目标 项目0014（L.C.史密斯）是确定机制， 甘油三酯被水解，并使用重组DNA方法来鉴定 血浆脂肪酶和apoC-II的功能区。 项目0009（L. Chan）使用转基因动物作为体内载体用于鉴定 VLDL和LDL中载脂蛋白B-100的功能区域。 所有 项目旨在确定异常的特殊作用， 甘油三酯代谢在动脉粥样硬化形成中的作用。 此外，（A） 行政核心，这些项目得到以下研究的支持 核心：（9005）细胞和分子生物学，（9004）肽测序和 氨基酸分析和（9002）脂蛋白分离和分析。

The overall theme of this application is the role of abnormal triglyceride and fatty acid metabolism in the etiology of atherosclerosis. Project 0006 (W. and J. Patsch) is on postprandial lipoproteins and has as its major goal identification of the molecular basis for the observed correlation between the duration of postprandial lipemia and the incidence of coronary artery disease. Project 0010 (J.B. Massey) investigates the role of lipid transfer and lipid transfer proteins on lipoprotein metabolism in vitro and in vivo. Project 0011 (H.J. Pownall) focuses on the mechanism and role of a plasma peptide that stimulates the formation of intracellular fatty acid esters. Both Projects 0010-0011 use the hamster as an animal model for diet-induced atherosclerosis. In Project 0012 (J.D. Morrisett), synthesis and catabolism of Lp[a] will be studied, with special emphasis given to the effects of postprandial lipemia on the formation of triglyceride-rich Lp[a] particles and their lipolysis in vivo. Project 0013: D.P. Via) is designed to determine how specific lipoproteins and their components regulate cell-specific cytokine production and secretion. The goal of Project 0014 (L.C. Smith) is to determine the mechanism by which plasma triglycerides are hydrolyzed and to use recombinant DNA methods to identify the functional regions of the plasma lipases and apoC-II. Project 0009 (L. Chan) uses transgenic animals as an in vivo vector for the identification of functional regions in apolipoprotein B-100 in VLDL and LDL. All projects are designed to identify the special role that abnormal triglyceride metabolism has in atherogenesis. In addition to an (A) Administrative Core, these projects are supported by the following research cores: (9005) Cell and Molecular Biology, (9004) Peptide Sequencing and Amino Acid Analysis, and (9002) Lipoprotein Isolation and Analysis.