权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Integrated transcriptome and genetic analysis of early events determining tissue susceptibility in the Claviceps purpurea - wheat interaction

决定麦角菌-小麦相互作用中组织易感性的早期事件的综合转录组和遗传分析

基本信息

批准号：
BB/G020418/1
负责人：
Donal O'Sullivan
金额：
$ 115.08万
依托单位：
National Institute of Agricultural Botany
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2009
资助国家：
英国
起止时间：
2009 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FG020418%2F1
关键词：
Integrated transcriptome genetic analysis early

项目摘要

The need for safe and nutritious wheat is as important as ever. The aim of this project is to gain an understanding of the interaction between the alkaloid producing plant pathogen Claviceps purpurea and its host hexaploid wheat. Claviceps forms a fungal body called an ergot in the place of a wheat grain following infection of the flower. Ergots contain high levels of extremely toxic alkaloids and pose a risk to humans and animals when ingested hence a zero tolerance level for wheat entering the human food chain and strict levels allowed for sale of grain for feed. Levels of ergots found in seed lots have been increasing over recent years which is driving the need for this research. In order to understand the fundamental biology of the interaction during the infection process, we will extract and measure relative abundance of the expressed genes from a set of very specific tissues of the infected wheat flower over a time course that identifies the key stages of Claviceps infection along with an uninfected control. Claviceps hyphae grow through the same tissues of the wheat flower as the growing pollen tube during pollination so we propose to include the pollen-flower interactions in the analysis. We will identify set of genes that are up and down regulated in response to either pollen and or hyphal growth and will identify common signals involved in perception and response. There is no chemical that can be deployed to defend against infection by Claviceps, so natural resistance to this pathogen must be exploited in any long-term sustainable control strategy. Partial resistance has been identified for a UK wheat variety and there is also source of near complete resistance identified in durum wheat, and we intend to locate the genes conferring these traits and to compare their location with those of genes whose expression appears to correlate in time and space with the expression of resistance. Additional evidence for or against the putative role of genes of interest in determining resistance will be gained by using genetic modification to increase or decrease expression of specific candidates.

对安全和营养小麦的需求与以往一样重要。本项目的目的是了解产生生物碱的植物病原菌麦角菌与其寄主六倍体小麦之间的相互作用。麦角菌在感染花后形成一个被称为麦角菌的真菌体，代替小麦粒。麦角含有高水平的剧毒生物碱，摄入后对人类和动物构成风险，因此对进入人类食物链的小麦的零容忍水平和饲料谷物的销售的严格水平。近年来，在种子中发现的麦角水平一直在增加，这推动了这项研究的需求。为了了解在感染过程中的相互作用的基本生物学，我们将提取和测量相对丰度的表达基因从一组非常具体的组织感染的小麦花在一个时间过程中，确定关键阶段的麦角菌感染沿着与未感染的控制。麦角菌菌丝生长通过相同的组织的小麦花在授粉过程中的生长的花粉管，所以我们建议包括花粉-花的相互作用的分析。我们将确定一组在花粉和/或菌丝生长中上调和下调的基因，并确定参与感知和反应的共同信号。没有化学物质可以用来防御麦角菌的感染，因此必须在任何长期可持续的控制策略中利用对这种病原体的天然抗性。部分抗性已被确定为英国小麦品种，也有来源的几乎完全的抗性鉴定硬质小麦，我们打算找到赋予这些性状的基因，并比较它们的位置与那些基因的表达似乎在时间和空间上与抗性的表达相关。通过使用基因修饰来增加或减少特定候选基因的表达，将获得支持或反对感兴趣的基因在确定抗性中的假定作用的额外证据。