The contribution of inner retinal photoreception to mouse visual function

视网膜内感光对小鼠视觉功能的贡献

• 批准号: BB/I007296/1
• 负责人: Robert Lucas
• 金额: $ 63.74万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FI007296%2F1
• 关键词: contribution; inner; retinal; photoreception; mouse

Sight is our most valued sense, and has been a perennial subject of scientific investigation over the centauries. Nonetheless, we continue to find out exciting new things about it. Among the most surprising of these has been that the rod and cone cells in the retina are not our only photoreceptors. In fact, we now know that another group of cells, called mRGCs, are capable of responding directly to light. These mRGCs work fairly well even when rods and cones are absent, and thus provide residual photosensitivity in some blind people. Until now, however, mRGCs have been thought to mainly provide input to crude 'sub conscious' responses to light, such as setting the size of the pupil in our eyes and ensuring that our internal biological clocks are set to local time. There has been little indication that they could also help us to see. We have recently shown that, in fact, signals from mRGCs appear throughout those parts of the brain responsible for visual perception. How then do mRGCs help us to see? Answering that question is the subject of this proposal. We will achieve this by providing visual stimuli capable of selectively activating mRGCs to several lines of transgenic mice. We will then be able to determine whether mice can use these stimuli to navigate a maze, and also what impact they have on the electrical activity of neurons in parts of the brain responsible for vision. Our work has the capacity to provide a new understanding of how we perceive the world. Because mRGCs survive in people with rod and cone degeneration, this information may be particularly pertinent for the blind and partially sighted.

视觉是我们最有价值的感觉，在过去的几个世纪里，它一直是科学研究的一个永恒主题。尽管如此，我们仍不断发现令人兴奋的新事物，其中最令人惊讶的是视网膜中的视杆细胞和视锥细胞并不是我们唯一的光感受器。事实上，我们现在知道另一组称为mRGCs的细胞能够直接对光线做出反应。这些mRGC即使在视杆细胞和视锥细胞不存在时也相当好地工作，因此在一些盲人中提供残余光敏性。然而，到目前为止，mRGCs一直被认为主要是为对光的原始“潜意识”反应提供输入，例如设置我们眼睛中瞳孔的大小，并确保我们的内部生物钟设置为当地时间。几乎没有迹象表明它们也可以帮助我们看到。事实上，我们最近已经证明，来自mRGCs的信号出现在负责视觉感知的大脑部分。那么mRGCs是如何帮助我们看到的呢？本提案的主题是解决这个问题。我们将通过提供能够选择性激活mRGC的视觉刺激来实现这一点。然后，我们将能够确定小鼠是否可以使用这些刺激来导航迷宫，以及它们对负责视觉的大脑部分神经元的电活动有什么影响。我们的工作有能力为我们如何看待世界提供新的理解。由于mRGC在杆和锥变性的人中存活，因此该信息可能与盲人和部分视力特别相关。

项目成果

Optogenetic interrogation reveals separable G-protein-dependent and -independent signalling linking G-protein-coupled receptors to the circadian oscillator.

• DOI: 10.1186/s12915-017-0380-8
• 发表时间: 2017-05-15
• 期刊: BMC biology
• 影响因子: 5.4
• 作者: Bailes HJ;Milosavljevic N;Zhuang LY;Gerrard EJ;Nishiguchi T;Ozawa T;Lucas RJ
• 通讯作者: Lucas RJ

Convergence of visual and whisker responses in the primary somatosensory thalamus (ventral posterior medial region) of the mouse.

• DOI: 10.1113/jp272791
• 发表时间: 2017-02-01
• 期刊: The Journal of physiology
• 作者: Allen AE;Procyk CA;Brown TM;Lucas RJ
• 通讯作者: Lucas RJ

Visual input to the mouse lateral posterior and posterior thalamic nuclei: photoreceptive origins and retinotopic order.

• DOI: 10.1113/jp271707
• 发表时间: 2016-04-01
• 期刊: The Journal of physiology
• 作者: Allen AE;Procyk CA;Howarth M;Walmsley L;Brown TM
• 通讯作者: Brown TM

Melanopsin-positive intrinsically photosensitive retinal ganglion cells: from form to function.

• DOI: 10.1523/jneurosci.4132-11.2011
• 发表时间: 2011-11-09
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Schmidt TM;Do MT;Dacey D;Lucas R;Hattar S;Matynia A
• 通讯作者: Matynia A

Measuring and using light in the melanopsin age.

• DOI: 10.1016/j.tins.2013.10.004
• 发表时间: 2014-01
• 期刊: Trends in neurosciences
• 影响因子: 15.9
• 作者: Lucas RJ;Peirson SN;Berson DM;Brown TM;Cooper HM;Czeisler CA;Figueiro MG;Gamlin PD;Lockley SW;O'Hagan JB;Price LL;Provencio I;Skene DJ;Brainard GC
• 通讯作者: Brainard GC