NMR PROTON CHEMICAL SHIFT IMAGING

核磁共振质子化学位移成像

• 批准号: 3180081
• 负责人: BRUCE R ROSEN
• 金额: $ 17.75万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-07-01 至 1988-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3180081
• 关键词: bone marrow disorder; brain neoplasms; cerebral ischemia /hypoxia; human subject; lactates; leukemia; lipids; liver disorder diagnosis; neoplasm /cancer radiodiagnosis; nuclear magnetic resonance spectroscopy; radiodiagnosis; stroke; water

The first nuclear magnetic resonance (NMR) images were produced in 1973; in the ensuing years significant advances have been made in instrumentation and technique. Concurrent with developments in imaging technology NMR spectroscopic techniques have been applied with increasing frequency to study biological systems. The goal of this grant is to further develop and evaluate techniques which acquire proton spectroscopic (chemical shift) information within an NMR image. Specifically, efforts will focus on two primary aims. First, the ability to measure signal intensity and relaxation times of the two primary contributors to the proton spectra, lipid and water, will be tested using three chemical shift imaging techniques. Phantom data will be used to verify theoretical predictions of S/N and technique bias. Rat models of hepatic steatosis with and without the concomitant hepatitis will be used to validate these observations in vivo, and to compare the sensitivity of conventional NMR techniques to the three chemical shift imaging methods using multicomponent relaxation time measurements. Studies will then be extended to the investigation of human liver pathology. In order to facilitate rapid transfer of information to the clinical environment, studies of human bone marrow will be made in parallel with animal liver studies. These will first focus on patterns of normal bone marrow development. A pilot study of bone marrow in human leukemias will then be carried out, testing the ability of multicomponent measurements to characterize normal and abnormal marrow elements. The second specific aim of this study is to use proton chemical shift imaging techniques to map cerebral lactate during global and focal hypoxic insult. The sensitivity, precision and accuracy of lactate measurements will be determined in phantoms, evaluating techniques of both water and lipid suppression. Non-imaging experiments in rats will be carried out to evaluate system linearity in vivo, and tissue lactate relaxation times will be determined over a range of concentrations. A cat brain model of global hypoxia, temporally stabalized, will be imaged, with brain lactate levels determined biochemically compared to lactate image signal intensity. This model wil be extended in studies of focal ischemic insult. Pilot studies of lactate accumulation in humans will also be carried out, investigating stroke and CNS tumor patients.

第一张核磁共振 (NMR) 图像于 1973 年生成；在 接下来的几年，仪器仪表方面取得了重大进展 和技术。 与 NMR 成像技术的发展同步 光谱技术的应用越来越频繁 研究生物系统。 这笔赠款的目标是进一步发展和 评估获取质子光谱（化学位移）的技术 NMR 图像中的信息。 具体而言，将重点做好两方面工作 主要目标。 首先，测量信号强度和 质子光谱的两个主要贡献者的弛豫时间， 脂质和水，将使用三种化学位移成像进行测试 技术。 虚拟数据将用于验证理论预测 信噪比和技术偏差。 有或没有脂肪变性的大鼠模型 伴随的肝炎将用于验证这些观察结果 体内，并比较传统 NMR 技术与 使用多组分弛豫时间的三种化学位移成像方法 测量。 然后研究将扩展到人类的调查 肝脏病理学。 为了方便信息的快速传递 在临床环境中，将对人类骨髓进行研究 与动物肝脏研究平行。 这些将首先集中于以下模式： 骨髓发育正常。 人类骨髓的初步研究 然后将进行白血​​病，测试多成分的能力 测量来表征正常和异常的骨髓元素。 这项研究的第二个具体目标是利用质子化学位移 成像技术绘制全身和局部缺氧期间的脑乳酸图 侮辱。 乳酸测量的灵敏度、精密度和准确度 将在模型中确定，评估水和水的技术 脂质抑制。 将在大鼠中进行非成像实验 评估体内系统线性度，组织乳酸弛豫时间将 可以在一定浓度范围内确定。 全球猫脑模型 缺氧，暂时稳定，将通过脑乳酸水平进行成像 与乳酸图像信号强度相比进行生化测定。 这 该模型将在局灶性缺血性损伤的研究中得到扩展。 试点研究 还将开展人体乳酸积累的研究，调查 中风和中枢神经系统肿瘤患者。