CLONING AND ANALYSIS OF THE VITAMIN K2 GENE CLUSTER

维生素K2基因簇的克隆与分析

• 批准号: 3286867
• 负责人: HARRY W TABER
• 金额: $ 10.13万
• 依托单位: ALBANY MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-30 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3286867
• 关键词: Bacillus subtilis; bacterial genetics; endonuclease; eukaryote; gel electrophoresis; gene complementation; genetic manipulation; genetic mapping; genetic recombination; genetic transcription; menadione; microorganism metabolism; molecular cloning; nucleic acid sequence; operon; radiotracer; regulatory gene; structural genes; vitamin K; vitamin biosynthesis

In humans and other higher animals, vitamin K (as vitamin K2 or menaquinone) serves as an essential cofactor in the formation of several clotting factors. The menaquinone-dependent biochemical reaction in clotting factor synthesis is the Gamma-carboxylation of glutamic acid. Among prokaryotic cells, on the other hand, menaquinone functions as a lipophilic electron carrier in membrane-bound electron transfer chains. Most bacterial species containing menaquinone are capable of synthesizing it de novo, and there is some evidence that intestinal flora can supply their animal hosts with menaquinone in the absence of dietary sources of the vitamin. Knowledge of the biosynthetic steps in bacterial menaquinone formation and the regulation of this biosynthesis should enhance our understanding of this aspect of bacterial-human interaction. The long-term objectives of the applicant's research program are: (i) to discover the principles governing regulations of bacterial menaquinone biosynthesis, both at the metabolic and the genetic levels; ii) to relate these principles to the functioning of menaquinone in the biology of prokaryotic cells. The specific aims of this research proposal are: i) the use of molecular cloning techniques to isolate from the bacterium Bacillus subtilis a cluster of genes (the men operon) encoding information for the menaquinone biosynthetic enzymes and associated regulatory regions; ii) the physical analysis of this DNA fragment by restriction mapping, subcloning, nucleotide sequence determination, and transcript mapping; iii) establishment of the presence, order, and functionality of structural genes within the operon fragment by recombination and complementation measurements of subcloned sequences.

在人类和其他高等动物中，维生素K(如维生素K2或

项目成果

Structure and expression of the cytochrome aa3 regulatory gene ctaA of Bacillus subtilis.

枯草芽孢杆菌细胞色素aa3调控基因ctaA的结构和表达。

• DOI: 10.1128/jb.171.9.4979-4986.1989
• 发表时间: 1989
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Mueller,JP;Taber,HW
• 通讯作者: Taber,HW

Transcriptional regulation of a promoter in the men gene cluster of Bacillus subtilis.

枯草芽孢杆菌 men 基因簇启动子的转录调控。

• DOI: 10.1128/jb.170.6.2742-2748.1988
• 发表时间: 1988
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Miller,P;Mueller,J;Hill,K;Taber,H
• 通讯作者: Taber,H

The Bacillus subtilis menCD promoter is responsive to extracellular pH.

枯草芽孢杆菌 menCD 启动子对细胞外 pH 值有反应。

• DOI: 10.1007/bf00249005
• 发表时间: 1990
• 期刊: Archives of microbiology
• 影响因子: 2.8
• 作者: Hill,KF;Mueller,JP;Taber,HW
• 通讯作者: Taber,HW

Isolation and sequence of ctaA, a gene required for cytochrome aa3 biosynthesis and sporulation in Bacillus subtilis.

ctaA 的分离和序列，ctaA 是枯草芽孢杆菌细胞色素 aa3 生物合成和孢子形成所需的基因。

• DOI: 10.1128/jb.171.9.4967-4978.1989
• 发表时间: 1989
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Mueller,JP;Taber,HW
• 通讯作者: Taber,HW

Molecular cloning and preliminary genetic analysis of the men gene cluster of Bacillus subtilis.

枯草芽孢杆菌men基因簇的分子克隆及初步遗传分析。

• DOI: 10.1128/jb.170.6.2735-2741.1988
• 发表时间: 1988
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Miller,P;Rabinowitz,A;Taber,H
• 通讯作者: Taber,H