Microneedle-mediated enhanced Raman therapeutic drug monitoring

微针介导的增强拉曼治疗药物监测

• 批准号: EP/H021647/1
• 负责人: Ryan Donnelly
• 金额: $ 41.72万
• 依托单位: Queen's University Belfast
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2010
• 资助国家: 英国
• 起止时间: 2010 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FH021647%2F1
• 关键词: Microneedle; mediated; enhanced; Raman; therapeutic

Over the past 3 decades there has been a dramatic increase in the number of people infected with human immunodeficiency virus and Mycobacterium tuberculosis, such that approximately 40 million are currently infected by HIV and 14 million are infected with tuberculosis. Strict compliance with prescribed drug treatment regimens is required for management of these diseases in individuals and control of their spread to others. Monitoring of compliance using conventional methods is problematic, particularly in the Developing World, where lack of resources and improper use of needles causes significant problems for society. Advances in medical treatment mean more premature neonates now survive. Due to their prematurity and the associated complications, these patients are frequently treated with multiple drugs. Direct blood sampling can, however, cause severe bruising or scarring and such patients also have very limited blood volumes, which prevents frequent sampling. One in five fatal car accidents in the UK are currently due to drivers driving under the use of illegal or prescription drugs. No appropriate roadside test currently exists. When considering these factors, in addition to the time-consuming nature and expense of routine therapeutic drug monitoring in hospitals, it is obvious that development of novel non- or minimally-invasive technologies that permit rapid and frequent ambulatory monitoring without drawing of blood is extremely important. In this project, we will investigate novel technology based on tiny needles that puncture the outer layer of skin without causing any pain or bleeding - the sensation is said to feel like a cat's tongue or sharkskin. These needles will then swell, turning into a jelly-like material that keeps the holes open and allow collection of skin fluid. As the drug content in the fluid in people's skin is very similar to that in their blood, we can use our microneedles, in combination with a sophisticated measurement technique, known as Surface-enhanced Raman Spectroscopy, to accurately monitor blood levels of drugs.Once developed, the technology described here will allow frequent routine monitoring of patients. Accordingly, drug- and exogenous substance-associated adverse events and complications arising from blood sampling will be prevented, to the benefit of patients Worldwide. In the UK, the NHS will benefit from reduced costs due to shorter hospital stays and reduced occurrence of inappropriate dosing. Ultimately, health-related-quality-of-life will be enhanced through improved disease control, rapid detection of dangerously high or low levels, facile monitoring of adherence to prescribed regimens (eg treatment of tuberculosis in the Developing World) and detection of illicit substances in addicts or vehicle drivers. Preterm neonates are likely to derive great benefit from the marked increase in monitoring frequency permitted, as are elderly patients being treated with multiple drugs. Ultimately, commercialisation of the technology will be the primary route by which UK industry, the NHS and patients will derive benefits. In order to attract potential industrial partners, it is vitally important to demonstrate proof of concept for this technology, which is the over-arching aim of the present proposal.

在过去30年中，感染人类免疫缺陷病毒和结核分枝杆菌的人数急剧增加，目前约有4000万人感染艾滋病毒，1400万人感染结核病。需要严格遵守规定的药物治疗方案，以便在个人中管理这些疾病并控制其向其他人的传播。使用常规方法监测遵守情况是有问题的，特别是在发展中国家，那里缺乏资源和不当使用针头给社会带来了重大问题。医疗的进步意味着现在有更多的早产儿存活下来。由于他们的早产儿和相关的并发症，这些患者经常使用多种药物治疗。然而，直接采血可能会导致严重的瘀伤或疤痕，而且这类患者的血量也非常有限，这阻碍了频繁采样。目前，英国五分之一的致命车祸是由于司机使用非法或处方药驾驶造成的。目前还不存在适当的路边测试。考虑到这些因素，除了医院常规治疗药物监测的耗时和费用外，很明显，开发新的非或微创技术，允许快速和频繁地动态监测而不抽血，是极其重要的。在这个项目中，我们将研究基于微小针头的新技术，这种针头可以刺穿皮肤的外层，而不会引起任何疼痛或出血--据说这种感觉就像猫的舌头或鲨鱼皮。然后，这些针会膨胀，变成一种果冻状的物质，保持孔洞打开，并允许收集皮肤液。由于人体皮肤液体中的药物含量与血液中的药物含量非常相似，我们可以使用我们的微针，结合一种名为表面增强拉曼光谱的复杂测量技术，来准确地监测药物的血液水平。一旦开发出来，这里描述的技术将允许对患者进行频繁的常规监测。因此，采血引起的药物和外源性物质相关的不良事件和并发症将得到预防，使全世界的患者受益。在英国，NHS将受益于因缩短住院时间和减少不适当剂量的发生而降低的成本。归根结底，与健康有关的生活质量将通过改进疾病控制、快速检测危险的高或低水平、方便地监测遵守规定疗法的情况(例如发展中国家的结核病治疗)以及检测吸毒者或车辆司机的非法物质而得到提高。早产儿可能会从允许的监测频率显著增加中获得巨大好处，接受多种药物治疗的老年患者也是如此。最终，这项技术的商业化将是英国工业界、英国国民健康保险制度和患者受益的主要途径。为了吸引潜在的工业伙伴，至关重要的是证明这项技术的概念，这是本提案的总体目标。

项目成果

Investigation of the chemical origin and evidential value of differences in the SERS spectra of blue gel inks.

• DOI: 10.1039/c6an00972g
• 发表时间: 2016-08
• 期刊: The Analyst
• 作者: Y. Ho;Wendy W. Y. Lee;S. Bell

Hydrogel-Forming Microneedle Arrays for Enhanced Transdermal Drug Delivery.

• DOI: 10.1002/adfm.201200864
• 发表时间: 2012-12-05
• 期刊: ADVANCED FUNCTIONAL MATERIALS
• 影响因子: 19
• 作者: Donnelly, Ryan F.;Singh, Thakur Raghu Raj;Garland, Martin J.;Migalska, Katarzyna;Majithiya, Rita;McCrudden, Cian M.;Kole, Prashant Laxman;Mahmood, Tuan Mazlelaa Tuan;McCarthy, Helen O.;Woolfson, A. David
• 通讯作者: Woolfson, A. David

Transdermal on-demand drug delivery based on an iontophoretic hollow microneedle array system.

• DOI: 10.1039/d3lc00160a
• 发表时间: 2023-04
• 期刊: Lab on a chip
• 影响因子: 6.1
• 作者: Usanee Detamornrat;Marc Parrilla;Juan Domínguez-Robles;Q. K. Anjani;E. Larrañeta;K. De Wael;R. Donnelly

Hydrogel-Forming Microneedle Arrays Allow Detection of Drugs and Glucose In Vivo: Potential for Use in Diagnosis and Therapeutic Drug Monitoring.

• DOI: 10.1371/journal.pone.0145644
• 发表时间: 2015
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Caffarel-Salvador E;Brady AJ;Eltayib E;Meng T;Alonso-Vicente A;Gonzalez-Vazquez P;Torrisi BM;Vicente-Perez EM;Mooney K;Jones DS;Bell SE;McCoy CP;McCarthy HO;McElnay JC;Donnelly RF
• 通讯作者: Donnelly RF

Microneedle array-based electrochemical sensor functionalized with SWCNTs for the highly sensitive monitoring of MDMA in interstitial fluid

• DOI: 10.1016/j.microc.2023.109257
• 发表时间: 2023-08-27
• 期刊: MICROCHEMICAL JOURNAL
• 影响因子: 4.8
• 作者: Dragan，Ana-Maria;Parrilla，Marc;De Wael，Karolien
• 通讯作者: De Wael，Karolien