PLASMA PROTEIN REACTIONS IN SEPARATED FLOWS

分离流中的血浆蛋白反应

• 批准号: 3363347
• 负责人: Edward F. Leonard
• 金额: $ 14.76万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3363347
• 关键词: biomaterial interface interaction; biomaterials; blood proteins; cardiovascular prosthesis; cell adhesion; clotting factor; fibrinogen; granulocyte; platelets; polyethylenes; protein metabolism; stainings; thrombosis

The overriding problem of understanding and controlling blood-surface reactions is inability to relate precise but limited information obtained from in vitro studies to the process that takes place when a cardiovascular prosthesis or artificial organ is employed. Work proposed here would link to clinical conditions well-established descriptions of how blood proteins in unmoving, highly dilute plasma solutions adsorb, react and desorb at biomaterial surfaces. The proposed work involves four capabilities: (1) Establishing "separated flows" in a convenient laboratory environment. (Within such flows, at the start of clinical procedures, whole blood is likely to be diluted before it contacts biomaterial surfaces, and such flows are suspected of favoring the initiation of thrombosis.) (2) Imaging patterns of protein deposition on surfaces which bound separated flows, utilizing metal oxide particles that have been rendered specific to designated proteins. (3) Using biological tests, in situ, for determining the reactivity of the adsorbed proteins. (4) Analyzing the geometric aspects of these patterns using a scanning device and computer workstation for image analysis. Conclusions drawn from this work would be confirmed by contacting surfaces with whole blood in the same separated-flow apparatus. Surfaces to be used include cleaned glass as well as the NHLBI reference biomaterials, polydimethylsiloxane and low-density polyethylene.

认识和控制血液表面的首要问题 反应是无法将获得的准确但有限的信息联系起来 从体外研究到心血管疾病发生的过程 使用假体或人造器官。这里提出的工作将把 对临床情况的良好描述血液蛋白是如何 在静止的、高度稀释的等离子体溶液中，吸附、反应和解吸 生物材料表面。拟议的工作涉及四个能力：(1) 在便利的实验室环境中建立“分流”。 (在这种流动中，在临床程序开始时，全血是 很可能在接触生物材料表面之前被稀释，等等 血流被怀疑有利于血栓的形成。)(2)成像 蛋白质在连接分离流动的表面上的沉积模式， 利用金属氧化物颗粒，这些颗粒被渲染为特定于 指定的蛋白质。(3)使用生物试验，在现场确定 被吸附的蛋白质的反应性。(4)几何分析 使用扫描设备和计算机工作站的这些图案的各方面 用于图像分析。从这项工作中得出的结论将由 在同一分流装置中与全血接触表面。 使用的表面包括清洁的玻璃以及NHLBI参考 生物材料、聚二甲基硅氧烷和低密度聚乙烯。