PHYSIOLOGY OF EXCITABLE MEMBRANES

可兴奋膜的生理学

• 批准号: 3393287
• 负责人: JOHN W MOORE
• 金额: $ 16.11万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-09-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3393287
• 关键词: Cephalopoda; anesthetics; axon; calcium channel; calcium metabolism; computer simulation; image processing; lizards; mathematical model; membrane potentials; neural conduction; neural facilitation; neural initiation; neural transmission; neurochemistry; neuromuscular junction; neurons; neuropharmacology; neurophysiology; nuclear magnetic resonance spectroscopy; synapses; temperature jump; videotape /videodisc; voltage /patch clamp

We plan to exploit newly developed possibilities to learn about the machinery of synaptic transmission by use of the neuromuscularjunction on the ceratomandibularis muscle of the lizard. We plan to use focal extracellular recordings and patch clamps at the terminal boutons of the nerve ending to: 1) determine the type (or types) of calcium channels and their location 2) determine the relationship between calcium current and transmitter release 3) determine the relationship between the sub-membrane Ca concentration and transmitter release 4) identify a putative Na-Ca exchange current 5) look for evidence of vesicular fusion by video contrast enhancement and image processing 6) test for insertion of new channels by fusion of vesicles into the surface membrane of the bouton. We will simulate our experiments with a powerful new computer program in order o gain insight into our interpretations of the currents, Ca concentration profiles, and vesicle fusion machinery.

我们计划利用新开发的可能性来了解 突触传递机制的使用， 角下颌肌神经肌肉接头 蜥蜴 我们计划使用局灶性细胞外记录和膜片钳， 神经末梢的终末终扣： 1)确定钙通道的类型（或类型）及其 位置 2)确定钙电流与 递质释放 3)确定膜下Ca 浓度和递质释放 4)确定假定Na-Ca交换电流 5)通过视频对比寻找囊泡融合的证据 增强与图像处理 6)囊泡融合插入新通道试验 也就是钮扣的表面膜。 我们将用一台功能强大的新计算机模拟我们的实验 计划，以便深入了解我们对 电流、Ca浓度分布和囊泡融合机制。