PHYSIOLOGY OF EXCITABLE MEMBRANES

我们计划利用新开发的可能性来了解 突触传递的机制通过使用 人颈上颌肌的神经肌肉连接 蜥蜴。 我们计划使用焦点细胞外记录和膜片钳在 神经末梢的末端通向： 1)确定钙通道的类型及其 位置 2)确定钙电流与钙离子的关系 发射机释放 3)确定膜下壳之间的关系 浓度和递质释放 4)确定可能的钠钙交换电流 5)通过视频对比寻找囊泡融合的证据 增强和图像处理 6)通过将小泡融合到 布顿的表面膜。 我们将用一台功能强大的新计算机来模拟我们的实验 计划，以便深入了解我们对 电流、钙浓度分布和囊泡融合机制。

We plan to exploit newly developed possibilities to learn about the machinery of synaptic transmission by use of the neuromuscularjunction on the ceratomandibularis muscle of the lizard. We plan to use focal extracellular recordings and patch clamps at the terminal boutons of the nerve ending to: 1) determine the type (or types) of calcium channels and their location 2) determine the relationship between calcium current and transmitter release 3) determine the relationship between the sub-membrane Ca concentration and transmitter release 4) identify a putative Na-Ca exchange current 5) look for evidence of vesicular fusion by video contrast enhancement and image processing 6) test for insertion of new channels by fusion of vesicles into the surface membrane of the bouton. We will simulate our experiments with a powerful new computer program in order o gain insight into our interpretations of the currents, Ca concentration profiles, and vesicle fusion machinery.