PHYSIOLOGY OF EXCITABLE MEMBRANES

可兴奋膜的生理学

• 批准号: 2260142
• 负责人: JOHN W MOORE
• 金额: $ 16.41万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-09-01 至 1995-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2260142
• 关键词: Cephalopoda; anesthetics; axon; calcium channel; calcium metabolism; computer simulation; image processing; lizards; mathematical model; membrane potentials; neural conduction; neural facilitation; neural initiation; neural transmission; neurochemistry; neuromuscular junction; neurons; neuropharmacology; neurophysiology; nuclear magnetic resonance spectroscopy; synapses; temperature jump; videotape /videodisc; voltage /patch clamp

We plan to exploit newly developed possibilities to learn about the machinery of synaptic transmission by use of the neuromuscularjunction on the ceratomandibularis muscle of the lizard. We plan to use focal extracellular recordings and patch clamps at the terminal boutons of the nerve ending to: 1) determine the type (or types) of calcium channels and their location 2) determine the relationship between calcium current and transmitter release 3) determine the relationship between the sub-membrane Ca concentration and transmitter release 4) identify a putative Na-Ca exchange current 5) look for evidence of vesicular fusion by video contrast enhancement and image processing 6) test for insertion of new channels by fusion of vesicles into the surface membrane of the bouton. We will simulate our experiments with a powerful new computer program in order o gain insight into our interpretations of the currents, Ca concentration profiles, and vesicle fusion machinery.

我们计划利用新开发的可能性来了解 突触传递机制 角下颌肌上的神经肌肉接头 蜥蜴。 我们计划使用焦点细胞外记录和膜片钳 神经末梢的末端按钮： 1）确定钙通道的类型及其 地点 2）确定钙电流与 发射机释放 3）确定亚膜Ca之间的关系 浓度和递质释放 4) 确定假定的 Na-Ca 交换电流 5）通过视频对比寻找囊泡融合的证据 增强和图像处理 6) 测试通过囊泡融合插入新通道 按钮的表面膜。 我们将用一台功能强大的新计算机来模拟我们的实验 计划以便深入了解我们对 电流、Ca 浓度分布和囊泡融合机制。

项目成果

Effects of the dipolar form of phloretin on potassium conductance in squid giant axons.

根皮素偶极形式对鱿鱼巨轴突钾电导的影响。

• DOI: 10.1016/s0006-3495(80)85053-3
• 发表时间: 1980
• 期刊: Biophysical journal
• 影响因子: 3.4
• 作者: Strichartz,GR;Oxford,GS;Ramon,F
• 通讯作者: Ramon,F

Potassium ion currents in the crayfish giant axon. Dynamic characteristics.

小龙虾巨轴突中的钾离子电流。

• DOI: 10.1016/s0006-3495(81)84761-3
• 发表时间: 1981
• 期刊: Biophysical journal
• 影响因子: 3.4
• 作者: Young,SH;Moore,JW
• 通讯作者: Moore,JW

Dynamics of potassium ion currents in squid axon membrane. A re-examination.

鱿鱼轴突膜中钾离子电流的动力学。

• DOI: 10.1016/s0006-3495(81)84760-1
• 发表时间: 1981
• 期刊: Biophysical journal
• 影响因子: 3.4
• 作者: Moore,JW;Young,SH
• 通讯作者: Young,SH