CRITICAL FLUID EXTRACTION AND PURIFICATION OF TAXOL

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Numerous compounds showing antineoplastic activity have been identified in the organic solvent extracts of plant material, microorganisms and marine organisms. Further investigation into the utility of these compounds as chemotherapeutic and anti-HIV agents has been hampered by their low natural abundance and the inefficiencies of conventional extraction techniques. An example of one such compound is taxol (NSC 125973), a diterpene plant product derived from the western yew Taxus brevifolia. The conventional organic phase extraction and chromatographic purification process for preparing taxol is time consuming and could also be harmful to the biologically active compounds because of their long residence time in a harsh environment. This technique is also encumbered by low overall yields. Our Phase I research indicated the application of supercritical fluid extraction and chromatography to the isolation and purification of taxol from yew needles to hold great promise. Potential advantages of this approach are: higher speed of extraction; improved production capacity; higher overall efficiency; and improved product quality.