CRITICAL FLUID EXTRACTION AND PURIFICATION OF TAXOL

紫杉醇的临界流体萃取和纯化

• 批准号: 3506914
• 负责人: TREVOR P. CASTOR
• 金额: $ 23.68万
• 依托单位: APHIOS CORPORATION
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1994-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3506914
• 关键词: Conifera; chromatography; diterpenes; drug design /synthesis /production; evaluation /testing; fluid; mathematical model; paclitaxel; plant extracts; solvent extraction; thermodynamics

Numerous compounds showing antineoplastic activity have been identified in the organic solvent extracts of plant material, microorganisms and marine organisms. Further investigation into the utility of these compounds as chemotherapeutic and anti-HIV agents has been hampered by their low natural abundance and the inefficiencies of conventional extraction techniques. An example of one such compound is taxol (NSC 125973), a diterpene plant product derived from the western yew Taxus brevifolia. The conventional organic phase extraction and chromatographic purification process for preparing taxol is time consuming and could also be harmful to the biologically active compounds because of their long residence time in a harsh environment. This technique is also encumbered by low overall yields. Our Phase I research indicated the application of supercritical fluid extraction and chromatography to the isolation and purification of taxol from yew needles to hold great promise. Potential advantages of this approach are: higher speed of extraction; improved production capacity; higher overall efficiency; and improved product quality.

许多具有抗肿瘤活性的化合物已在 植物材料、微生物和海洋的有机溶剂提取物 有机体。进一步研究这些化合物作为 化疗和抗艾滋病毒药物因其天然成分低而受到阻碍。 丰度和传统提取技术的低效。一个 一种这样的化合物的例子是紫杉醇(NSC 125973)，一种二萜植物 产品来源于西部短叶红豆杉。 常规有机相萃取和层析纯化 制备紫杉醇的过程非常耗时，而且可能对 具有生物活性的化合物，因为它们在体内停留时间很长 恶劣的环境。这项技术也受到总体低成本的阻碍 收益率。我们的第一阶段研究表明超临界流体的应用 流体萃取和层析技术在分离纯化中的应用 紫杉醇从红豆杉针叶中提取，蕴含着巨大的希望。这样做的潜在优势 途径有：提高采掘速度；提高生产能力； 整体效率更高，产品质量更高。