Aluminium nitride - graphene dual-mode sensors for cancer cell detection

用于癌细胞检测的氮化铝-石墨烯双模式传感器

• 批准号: EP/P02985X/1
• 负责人: Norbert Klein
• 金额: $ 147.57万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FP02985X%2F1
• 关键词: Aluminium; nitride; graphene; dual; mode

We propose to develop a technology for wafer scale device fabrication based on thin film heterostructures composed of graphene and aluminium nitride (AlN), which were recently demonstrated by our team for the first time. As a new orthogonal approach for biosensors, AlN-graphene heterostructures should enable simultaneous mass and charge detection of immobilized cellular and molecular species within one integrated device. This new capability is expected to lead to a dramatic reduction of false positives and false negatives in cancer cell detection based on antigen-antibody bond-mediated cell capturing. : As a bioactive sensor surface, bio-functionalized graphene enables charge detection via the electric field effect in graphene. Simultaneously, the graphene biointerface layer will be employed as an electrode - in combination with other graphene layer(s) embedded inside the heterostructure - to enable a fully integrated piezoelectric excitation and readout of mechanical deformations or oscillations of a free-standing AlN structure. Cell capture-induced alterations of the deformation or mechanical resonance should allow mass detection of the immobilized species. This orthogonal sensing capability of fully integrated devices will be tested and benchmarked for detection of cancer cells in blood or other body fluids.

我们建议开发一种基于石墨烯和氮化铝（AlN）组成的薄膜异质结构的晶圆级器件制造技术，这是我们团队最近首次展示的。作为一种新的正交生物传感器的方法，AlN-石墨烯异质结构应该能够在一个集成器件内同时检测固定的细胞和分子物种的质量和电荷。这种新的能力预计将导致基于抗原-抗体键介导的细胞捕获的癌细胞检测中的假阳性和假阴性的显著减少。作为生物活性传感器表面，生物功能化石墨烯通过石墨烯中的电场效应实现电荷检测。同时，石墨烯生物界面层将用作电极-与嵌入异质结构内部的其他石墨烯层组合-以实现完全集成的压电激励和独立AlN结构的机械变形或振荡的读出。细胞捕获诱导的变形或机械共振的改变应允许固定物种的质量检测。完全集成的设备的这种正交传感能力将被测试和基准化，用于检测血液或其他体液中的癌细胞。

项目成果

High-resolution, spatially-resolved surface potential investigations of high-strength metallurgical graphene using scanning tunnelling potentiometry

• DOI: 10.1016/j.mee.2019.03.023
• 发表时间: 2019-05
• 期刊: Microelectronic Engineering
• 影响因子: 2.3
• 作者: K. Gajewski;P. Kunicki;A. Sierakowski;W. Szymański;W. Kaczorowski;P. Niedzielski;S. Ramadan;O. Shaforost;N. Klein;L. Hao;T. Gotszalk

Enhancing Structural Properties and Performance of Graphene-Based Devices Using Self-Assembled HMDS Monolayers.

• DOI: 10.1021/acsomega.0c05631
• 发表时间: 2021-02-23
• 期刊: ACS omega
• 影响因子: 4.1
• 作者: Ramadan S;Zhang Y;Tsang DKH;Shaforost O;Xu L;Bower R;Dunlop IE;Petrov PK;Klein N
• 通讯作者: Klein N

Large-Area Electrodeposition of Few-Layer MoS2 on Graphene for 2D Material Heterostructures.

• DOI: 10.1021/acsami.0c14777
• 发表时间: 2020-10
• 期刊: ACS applied materials & interfaces
• 影响因子: 9.5
• 作者: Yasir J Noori;Shibin Thomas;S. Ramadan;Danielle E. Smith;V. Greenacre;N. Abdelazim;Yisong Han

Detection of Glial Fibrillary Acidic Protein in Patient Plasma Using On-Chip Graphene Field-Effect Biosensors, in Comparison with ELISA and Single-Molecule Array.

• DOI: 10.1021/acssensors.1c02232
• 发表时间: 2022-01-28
• 期刊: ACS sensors
• 影响因子: 8.9
• 作者: Xu L;Ramadan S;Akingbade OE;Zhang Y;Alodan S;Graham N;Zimmerman KA;Torres E;Heslegrave A;Petrov PK;Zetterberg H;Sharp DJ;Klein N;Li B
• 通讯作者: Li B

Microscale surface potential gradient disturbances observed in bilayer graphene

• DOI: 10.1016/j.apsusc.2020.145504
• 发表时间: 2020-04
• 期刊: Applied Surface Science
• 影响因子: 6.7
• 作者: K. Gajewski;S. Ramadan;P. Kunicki;O. Shaforost;N. Klein;T. Gotszalk