Extreme volumetric imaging using single-shot optical tomography with compressive sensing

使用具有压缩传感功能的单次光学断层扫描进行极限体积成像

基本信息

  • 批准号:
    EP/V048996/1
  • 负责人:
  • 金额:
    $ 25.63万
  • 依托单位:
  • 依托单位国家:
    英国
  • 项目类别:
    Research Grant
  • 财政年份:
    2021
  • 资助国家:
    英国
  • 起止时间:
    2021 至 无数据
  • 项目状态:
    已结题

项目摘要

Standard approaches to 3D imaging typically rely on taking a series of 2D images one after another and reconstructing the 3D volume. This puts an upper limit on the volumetric frame rate achievable, since it requires a certain amount of time to record the 10s-1000s of individual 2D images separately. This project aims to develop an imaging platform capable of full 3D volumetric imaging at acquisition rates exceeding 1000 volumes per second by acquiring multiple images in parallel using cost-effective high-speed cameras.The approach is based on the use of 4 high-speed cameras capable of frame rates in excess of 1000 frames per second. Previously, cameras that could operate at such high frame rates were prohibitively expensive, but recent advances in the technology has seen their price reduce significantly and brought them in line with the cost of more standard scientific cameras. Each camera will be combined with a novel optical system that produces two side-by-side images on the camera sensor, each viewing the sample at a slightly different angle. The four cameras will be placed around the sample and acquire eight images from different directions simultaneously, running at 1000s of frames a second. Each set of 8 images can them be combined to reconstruct the 3D scene, producing a 3D data set running at 1000s of volumes per second.The particular arrangement of the cameras and the approach to reconstruction will depend on the sample being imaged. In this project we will apply this extreme volumetric imaging system to two experimental investigations. To image semi-transparent biological samples a technique analogous to X-ray computed tomography, called optical projection tomography (OPT), will be applied. While typical OPT data sets require 100s of angularly resolved images, by applying advances computational analysis techniques we will be able to reconstruct the volume from the eight images acquired. This will permit OPT imaging of samples that have not been immobilized (e.g. no need for anesthesia) and can measure very fast biological processes in 3D, such as transient cell signaling events.While the use of high-speed cameras has been limited in biological imaging, they have been routinely used in fluid dynamics experiments to capture very fast events (e.g. droplet-surface interactions). We will extend this to full 3D imaging of such interactions by imaging them from eight different directions simultaneously and performing 3D reconstructions for every set of images (i.e. running at 1000s of volumes per second). For single droplet interactions we will employ surface measurement and reconstruction techniques, while for sprays (i.e. large number of droplets) we will employ particle tracking and optical scattering to quantify the trajectories and size of all the droplets. These experiments will be used to confirm and develop computational fluid dynamic simulations.
3D成像的标准方法通常依赖于一个接一个地拍摄一系列2D图像并重建3D体积。这对可实现的体积帧速率设置了上限,因为它需要一定量的时间来单独记录10秒至1000秒的单个2D图像。该项目旨在开发一种成像平台,能够以超过每秒1000个体积的采集速率通过使用具有成本效益的高速相机并行采集多个图像来进行全3D体积成像。该方法基于使用4台能够超过每秒1000帧的帧速率的高速相机。以前,能够以如此高的帧速率运行的相机非常昂贵,但该技术的最新进展使其价格显着降低,并使其与更标准的科学相机的成本保持一致。每个摄像头将与一个新颖的光学系统相结合,该系统在摄像头传感器上产生两个并排的图像,每个图像以略微不同的角度观察样品。这四台相机将被放置在样品周围,并同时从不同方向采集八张图像,每秒运行1000帧。每组8张图像可以组合起来重建3D场景,产生每秒1000个体积的3D数据集。相机的特定布置和重建方法将取决于被成像的样品。在这个项目中,我们将应用这种极端的体积成像系统的两个实验研究。为了对半透明的生物样品进行成像,将应用类似于X射线计算机断层扫描的技术,称为光学投影断层扫描(OPT)。虽然典型的OPT数据集需要100秒的角度分辨图像,但通过应用先进的计算分析技术,我们将能够从采集的8张图像中重建体积。这将允许OPT成像的样品还没有被固定(例如,不需要麻醉),并可以测量非常快速的生物过程中的3D,如瞬态细胞信号事件。虽然使用高速相机已被限制在生物成像,他们已被常规用于流体动力学实验,以捕捉非常快速的事件(例如液滴表面相互作用)。我们将通过同时从八个不同方向对它们进行成像并对每组图像进行3D重建(即以每秒1000个体积运行),将其扩展到这种相互作用的全3D成像。对于单个液滴相互作用,我们将采用表面测量和重建技术,而对于喷雾(即大量液滴),我们将采用颗粒跟踪和光学散射来量化所有液滴的轨迹和大小。这些实验将用于确认和开发计算流体动力学模拟。

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

James McGinty其他文献

Erratum to: Accelerated Gastric Emptying but No Carbohydrate Malabsorption 1 Year After Gastric Bypass Surgery (GBP)
  • DOI:
    10.1007/s11695-013-0965-4
  • 发表时间:
    2013-05-08
  • 期刊:
  • 影响因子:
    3.100
  • 作者:
    Gary Wang;Keesandra Agenor;Justine Pizot;Donald P. Kotler;Yaniv Harel;Bart J. Van Der Schueren;Iliana Quercia;James McGinty;Blandine Laferrère
  • 通讯作者:
    Blandine Laferrère
Hyperspectral scanning laser optical tomography
  • DOI:
    https://doi.org/10.1002/jbio.201800221
  • 发表时间:
    2018
  • 期刊:
  • 影响因子:
    2.8
  • 作者:
    Lingling Chen;Guiye Li;Li Tang;Meng Zhang;Lina Liu;Ang Liu;James McGinty;Shuangchen Ruan
  • 通讯作者:
    Shuangchen Ruan
Multiplexed Time Lapse Fluorescence Lifetime Readouts in an Optically Sectioning Time-Gated Imaging Microscope
  • DOI:
    10.1016/j.bpj.2010.12.1221
  • 发表时间:
    2011-02-02
  • 期刊:
  • 影响因子:
  • 作者:
    Anca Margineanu;Romain Laine;Sunil Kumar;Clifford Talbot;Sean Warren;Christopher Kimberley;James McGinty;Gordon Kennedy;Alessandro Sardini;Christopher Dunsby;Mark A.A. Neil;Matilda Katan;Paul M.W. French
  • 通讯作者:
    Paul M.W. French
In Vivo Investigation of Calpain Activity by Lifetime Imaging of Genetically Encoded FRET Sensors
  • DOI:
    10.1016/j.bpj.2011.11.866
  • 发表时间:
    2012-01-31
  • 期刊:
  • 影响因子:
  • 作者:
    Alessandro Sardini;Daniel W. Stuckey;James McGinty;Romain Laine;Vadim Y. Soloviev;Simon R. Arridge;Dominic J. Wells;Paul M.W. French;Joseph V. Hajnal
  • 通讯作者:
    Joseph V. Hajnal
P-26 Laparoendoscopic single-site surgery for sleeve gastrectomy: initial experience
  • DOI:
    10.1016/j.soard.2011.04.027
  • 发表时间:
    2011-05-01
  • 期刊:
  • 影响因子:
  • 作者:
    Koji Park;John N. Afthinos;James McGinty;Ninan Koshy;Julio Teixeira
  • 通讯作者:
    Julio Teixeira

James McGinty的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('James McGinty', 18)}}的其他基金

Enhancing spatial and temporal resolution for isotropic volumetric imaging and 3D cell tracking
增强各向同性体积成像和 3D 细胞跟踪的空间和时间分辨率
  • 批准号:
    BB/L018039/1
  • 财政年份:
    2014
  • 资助金额:
    $ 25.63万
  • 项目类别:
    Research Grant

相似海外基金

Concurrent volumetric imaging with multimodal optical systems
多模态光学系统的并行体积成像
  • 批准号:
    10727499
  • 财政年份:
    2023
  • 资助金额:
    $ 25.63万
  • 项目类别:
BRAIN CONNECTS: Center for a pipeline of high throughput integrated volumetric electron microscopy for whole mouse brain connectomics
大脑连接:用于全小鼠大脑连接组学的高通量集成体积电子显微镜管道中心
  • 批准号:
    10665386
  • 财政年份:
    2023
  • 资助金额:
    $ 25.63万
  • 项目类别:
Rapid Free-Breathing 3D High-Resolution MRI for Volumetric Liver Iron Quantification
用于体积肝铁定量的快速自由呼吸 3D 高分辨率 MRI
  • 批准号:
    10742197
  • 财政年份:
    2023
  • 资助金额:
    $ 25.63万
  • 项目类别:
Volumetric Real-Time MRI at 0.55 Tesla
0.55 特斯拉的体积实时 MRI
  • 批准号:
    10611241
  • 财政年份:
    2023
  • 资助金额:
    $ 25.63万
  • 项目类别:
Real-time Volumetric Imaging for Motion Management and Dose Delivery Verification
用于运动管理和剂量输送验证的实时体积成像
  • 批准号:
    10659842
  • 财政年份:
    2023
  • 资助金额:
    $ 25.63万
  • 项目类别:
Mesoscopic microscopy for ultra-high speed and large-scale volumetric brain imaging
用于超高速和大规模脑体积成像的介观显微镜
  • 批准号:
    10634911
  • 财政年份:
    2023
  • 资助金额:
    $ 25.63万
  • 项目类别:
Volumetric analysis of epithelial morphogenesis with high spatiotemporal resolution
高时空分辨率上皮形态发生的体积分析
  • 批准号:
    10586534
  • 财政年份:
    2023
  • 资助金额:
    $ 25.63万
  • 项目类别:
Volumetric imaging and computation to characterize cardiac electromechanical coupling
体积成像和计算来表征心脏机电耦合
  • 批准号:
    10629905
  • 财政年份:
    2023
  • 资助金额:
    $ 25.63万
  • 项目类别:
Sugar Probed SRS Volumetric imaging of Metabolic Activities
代谢活动的糖探针 SRS 体积成像
  • 批准号:
    10639208
  • 财政年份:
    2023
  • 资助金额:
    $ 25.63万
  • 项目类别:
Pediatric volumetric ultrasound scanner
儿科体积超声扫描仪
  • 批准号:
    10739411
  • 财政年份:
    2023
  • 资助金额:
    $ 25.63万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了