CORE--CELL SORTING UNIT

核心--细胞分选单元

• 批准号: 3729079
• 负责人: ROSS S BASCH
• 金额: --
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3729079
• 关键词: biomedical facility; cell sorting; human tissue; neoplastic cell

The Cell Sorting Unit makes available state-of-the-art flow technology service and training to Kaplan Comprehensive Cancer Center members. These services are designed to facilitate the research of the member, educate them about the technological possibilities offered by flow techniques and train pre- and post-doctoral students in the use of this technology. P30CA160879010 The Transgenic Mouse Research Facility provides a resource for Kaplan Comprehensive Cancer Center members seeking to generate transgenic mice to study various aspects of gene regulation. Users of the resource supply DNA constructs prepared in a manner suitable for injection into fertilized mouse eggs. Potential founder animals are produced and the tails removed by the resource facility and provided to the research investigator for DNA analysis by Southern blotting to determine integration-positive mice. Animals which are integration-positive are then handed over to the individual investigators to breed and study the effect of the expression of the injected DNA construct.

细胞分选单元提供最先进的流动技术 为Kaplan综合癌症中心成员提供服务和培训。这些 服务旨在促进会员的研究，教育 向他们介绍流动技术提供的技术可能性， 培训博士前和博士后学生使用这一技术。 P30CA160879010 转基因小鼠研究机构为卡普兰提供了一个资源 综合癌症中心成员寻求产生转基因小鼠 来研究基因调控的各个方面。资源供应的使用者 以适合于注射入的方式制备的DNA构建体 受精的老鼠卵潜在的创始人动物被生产出来， 由资源设施清除并提供给研究的尾矿 研究人员通过Southern印迹进行DNA分析，以确定 整合阳性小鼠。整合阳性的动物是 然后交给个别调查人员进行繁殖和研究， 注射的DNA构建体的表达的影响。