TRANSMISSION ELECTRON MICROSCOPE

透射电子显微镜

• 批准号: 2283826
• 负责人: DAVID S WILLIAMS
• 金额: $ 10万
• 依托单位: TRUSTEES OF INDIANA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-16 至 1995-05-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2283826
• 关键词: biomedical equipment purchase; cell biology; molecular biology; neurobiology; transmission electron microscopy

A current model transmission electron microscope (TEM) is requested for use in a central campus multi-user facility. The TEMs presently being used were both purchased in the l96Os, and have become inadequate for our present needs. They do not provide some of the functions required by some of the major users. Repair and maintenance have become severe problems, with disruptive "down-time" a common occurrence. The need for an efficient multi-user instrument has become particularly urgent due to a significant recent increase in the number of researchers in a variety of Departments on campus now employing TEM in their research. The requested model TEM, a JEM 1010, has the required functions that our present TEMs lack -specimen tilt, high contrast, minimum dose system - as well as a number of desired improvements in image quality and manipulation. The user-friendliness of the TEM makes it suitable as an efficient multi-user instrument; it would be housed in a new EM suite in the newly-constructed Institute for Molecular and Cellular Biology building, and thus be available as a central campus facility. Given their unreliability alone, neither of the present TEMs would be suitable for this facility. Centralization of our EM facility will optimize our resources, such as technical personnel, and provide better access to TBM for researchers from Departments that do not have a TEM. The requested grant is for just over half of the cost of the TEM; Indiana University will pay for the balance, as well as for a full service contract and the salary of a full-time EM technician. The user group is made up of ten different research labs in five different Departments. The NIH-funded projects that would use the new TEM involve the study of: (i) Cellular processes in photoreceptors; (ii) Control of microtubule function in development; (iii) Mechanisms and functions of subcellular motility; (iv) Analysis of protein kinase C's role in learning in Hermissenda; (v) Neurogenesis in Drosophila; (vi) Protein kinase C in photoreceptors; (vii) Steroids and trophic factors in an aging neuromuscular system; and (viii) Radiation repair and meiosis in Coprinus. Two projects for which NIH funding is being sought are: (i) Control of asymmetry in cell division during development; and (ii) Ocular toxicity studies involving the corneal epithelium.

当前的模型传输电子显微镜（TEM） 在中央校园多用户设施中使用。目前使用的TEMS 都在L96OS中购买，并且已经不足 目前的需求。他们不提供某些功能 主要用户。维修和维护已成为严重的问题， 具有破坏性的“停机时间”。需要有效的 由于重要的 最近的各个部门研究人员数量增加 在校园里，现在在他们的研究中使用TEM。请求的模型TEM，一个 JEM 1010，具有我们目前缺乏的所需功能 倾斜，高对比度，最低剂量系统 - 以及许多所需的 图像质量和操纵的改进。用户友好 TEM使其适合作为有效的多用户仪器；会 被安置在新建设的研究所的新EM套房中 分子和细胞生物学建筑，因此可作为 中央校园设施。鉴于他们的不可靠性，这两个都不是 目前的TEMS适合此设施。我们EM的集中化 设施将优化我们的资源，例如技术人员，以及 为无法的部门的研究人员提供更好的TBM访问权限 有一个tem。所请求的赠款仅占成本的一半以上 tem;印第安纳大学将支付余额，并全额支付 服务合同和全职EM技术人员的薪水。用户 小组由五个不同的十个不同研究实验室组成 部门。 NIH资助的将使用新TEM的项目涉及 研究：（i）感光细胞中的细胞过程； （ii）控制 开发中的微管功能； （iii）机制和功能 亚细胞运动； （iv）分析蛋白激酶C在学习中的作用 在Hermissenda； （v）果蝇中的神经发生； （VI）蛋白激酶C 感受器； （vii）衰老的类固醇和营养因子 神经肌肉系统； （viii）Coprinus的辐射修复和减数分裂。 寻求NIH资金的两个项目是：（i）控制 发育过程中细胞分裂的不对称性； （ii）眼毒性 涉及角膜上皮的研究。