SCREENING BRAIN DNA FOR HTLV-1 PROVIRAL SEQUENCES USING PCR AMPLIFICATION

使用 PCR 扩增筛选脑 DNA 中的 HTLV-1 原病毒序列

• 批准号: 3781410
• 负责人: D GLOVINSKY
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3781410
• 关键词: brain; genetic transcription; human T cell lymphotropic virus type 1; human genetic material tag; human tissue; multiple sclerosis; nervous system infection; neurotropic virus; nucleic acid sequence; polymerase chain reaction; postmortem; provirus; virus DNA

Polymerase chain reaction (PCR) amplification has been used to identify retrovirus-like sequences in both multiple sclerosis (MS) patients and non-neurologic-control patients. Having used PCR to identify a novel endogenous retrovirus-like sequence on human brain DNA, we are now defining its tissue distribution and transcriptional activity. Genomic DNA was extracted from 12 autopsied brains of patients with multiple sclerosis and from eight without MS. PCR amplification of the DNA was performed, and radiolabeled fragments of cloned sequences were used to probe DNA complementary to RNA from different human organs, as well as from various animals. No correlation between PCR amplification and MS was found. The DNA insert cloned from PCR amplification products differed from any previously described sequence, and most likely represents an endogenous DNA sequence.

聚合酶链反应（PCR）扩增已用于鉴定 逆转录病毒样序列在多发性硬化（MS）患者和 非神经系统控制患者。 利用PCR技术鉴定了一种新的 内源性逆转录病毒样序列的人脑DNA，我们现在 定义其组织分布和转录活性。 基因组 从12例多发性硬化症患者的尸检大脑中提取DNA， 硬化和8个没有MS。PCR扩增的DNA是 进行，并使用克隆序列的放射性标记片段， 与来自不同人体器官的RNA互补的探针DNA，以及 从各种动物。 PCR扩增与MS无相关性 从PCR扩增产物中克隆到了DNA插入片段 与之前描述的序列不同， 代表内源性DNA序列。