BACTERIAL FUNCTION INVOLVED IN CELL GROWTH CONTROL

参与细胞生长控制的细菌功能

• 批准号: 3796484
• 负责人: S GOTTESMAN
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3796484
• 关键词: bacterial capsules; bacterial genetics; bacterial polysaccharides; carbohydrate biosynthesis; cell cycle; cell growth regulation; endopeptidases; enzyme induction /repression; enzyme mechanism; enzyme substrate; gene expression; molecular chaperones; phosphorylation; protein biosynthesis; protein degradation; regulatory gene; transcription factor

We have been studying the role that protein degradation plays in regulating gene expression and have continued with studies on the linkages between chromosome synthesis and partition of chromosomes during cell division. As part of continuing studies on the regulation of capsular polysaccharide synthesis by the unstable regulator RcsA, we have found that a downstream transacting regulator of rcsA synthesis encodes RNAs which apparently mediate the regulatory effect. Mutations in both the regulator and target will allow further characterization of the mode of regulation. Mutations in the putative phosphorylation site of RcsB support a role for phosphorylation in capsule regulation. We are investigating the role of the heat shock chaperone proteins in degradation of substrates of the Lon ATP-dependent protease and initiating studies on the recognition specificity of Lon. Studies on the role and regulation of the Clp energy- dependent protease have concentrated on developing strains for the tight regulation of Clp synthesis, and investigating the function of a newly discovered alternate regulatory subunit, ClpX.

我们一直在研究蛋白质降解在调节 基因表达，并继续研究基因表达之间的联系 在细胞分裂过程中染色体的合成和分配。 作为 一部分继续研究的调节荚膜多糖 通过不稳定调节剂RcsA的合成，我们发现， rcsA合成的反式调节子编码RNA， 介导调节效应。 调节因子和靶基因的突变 将允许进一步表征监管模式。 突变 在RcsB的假定磷酸化位点中， 胶囊调节中的磷酸化。 我们正在调查 热激分子伴侣蛋白在Lon降解底物中的作用 ATP依赖性蛋白酶及其识别的初步研究 Lon的特性 Clp能量的作用及其调节的研究 依赖蛋白酶的人集中精力开发菌株， Clp合成的调节，并研究一种新的 发现了另一种调节亚基ClpX。