BACTERIAL FUNCTION INVOLVED IN CELL GROWTH CONTROL

参与细胞生长控制的细菌功能

• 批准号: 3808540
• 负责人: S GOTTESMAN
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3808540
• 关键词: Escherichia coli; bacterial genetics; biological signal transduction; cell capsule; cell cycle; cell growth regulation; chromosome translocation; enzyme induction /repression; gene expression; genetic mapping; molecular genetics; plasmids; protein biosynthesis; protein degradation; proteolysis; regulatory gene

We have been studying the role that protein degradation plays in regulating gene expression and have initiated a study on the linkages between chromosome synthesis and partition of chromosomes during cell division. We have continued our study of the mechanism of regulation of capsule synthesis, as a model for understanding the role of unstable proteins as regulators. RcsA, a target for the Lon protease, was shown to interact with another regulator, RcsB, in a temperature sensitive manner. Studies on rcsA expression have demonstrated a trans-acting role for a site downstream of the structural gene in regulating rcsA. We have continued studies on a new proteolytic activity capable of degading the Lon substrates, RcsA and SulA, which appears when a multicopy plasmid carrying a gene we have called alp is introduced into cells. The new activity is dependent on excision of a cryptic prophage and consequent inactivation of a chromosomal gene encoding a stable RNA. A third energy-dependent protease under study is the two component Clp protease. We are continuing mutagenesis studies of the CIpP subunit to determine the specificity and role of CIpP-dependent CIpP processing for assembly and proteolytic activity. In a separate project, camphor resistant mutations in four different loci have been shown by flow cytometry to lead to increased ploidy. In combination with previous genetic analysis, this suggests these mutations (called mbr) define three different coupling events for chromosome number and cell cycle.

我们一直在研究蛋白质降解在 调节基因表达，并启动了一项关于 细胞分裂过程中染色体的合成和分配 师. 我们继续研究了 胶囊合成，作为一个模型，了解不稳定的作用， 蛋白质作为调节剂。 显示了Lon蛋白酶的靶标RcsA， 与另一个调节器RcsB相互作用， 方式 对rcsA表达的研究表明， 在调节rcsA的结构基因下游的位点。 我们有 继续研究一种新的蛋白水解活性， Lon底物，RcsA和苏拉，当多拷贝质粒 携带一种我们称之为ALP的基因被引入细胞。 新 活性依赖于隐蔽原噬菌体的切除， 编码稳定RNA的染色体基因失活。 第三 所研究的能量依赖性蛋白酶是双组分Clp蛋白酶。 我们正在继续进行ClpP亚基的诱变研究，以确定 依赖于ClpP的ClpP加工对组装的特异性和作用 和蛋白水解活性。 在另一个项目中， 流式细胞术显示，四个不同基因座中的突变 导致倍性增加。 结合之前的基因分析， 这表明这些突变（称为mbr）定义了三种不同的 染色体数目和细胞周期的偶联事件。