IMMUNOLOGICAL STUDIES IN CHRONIC VIRAL HEPATITIS

免疫因素似乎在决定病程和 急性和慢性病毒性肝炎的转归。此外，前景看好 慢性病毒性肝炎的治疗对免疫功能有深远的影响 对治疗的功能和持续反应可能在很大程度上取决于 恢复正常的免疫反应。免疫学的作用 决定病毒病程和最终结局的机制 肝炎正在研究中。用逆转录法测定细胞因子mRNAs 转录酶聚合酶链式反应技术在14例肝细胞癌患者肝活检中的应用 HBeAg阳性慢性乙型肝炎和6例正常人(移植 捐赠者)。7例对照组织中只有1例呈阳性。 检测mRNAs。相比之下，有很高的患病率(43-80%) 慢性乙型肝炎患者外周血中IL-1、IL-2、IL-4和干扰素-γ的检测 IL-5、IL-6和TNF-α的mRNAs在慢性肝炎中不常见 B.定量RT-PCR显示高水平的IL-4和干扰素-4的mRNA 伽马与严重的炎症有关，这表明 IL-4和干扰素-γ在大鼠肝组织损伤中可能起重要作用 病毒复制活跃的慢性乙肝。

Immunological factors seem to be important in determining the course and outcomes of both acute and chronic viral hepatitis. Furthermore, promising therapies for chronic viral hepatitis have profound effects on immune function and sustained responses to therapy may depend largely on restoration of normal immune responsiveness. The role of immunologic mechanisms in determining the course and ultimate outcome of viral hepatitis is being studied. Cytokine mRNAs were determined using a reverse transcriptase (RT) PCR technique in liver biopsies from 14 patients with HBeAg positive chronic hepatitis B and 6 normal subjects (transplantation donors). Only one of the control biopsies was positive for 2 of the 7 mRNAs assayed. In contrast, there was a high prevalence (43-80%) of detectable mRNAs for IL-1, IL-2, IL-4 and IFN-gamma in chronic hepatitis B. mRNAs for IL-5, IL-6 and TNF-alpha were not prevalent in chronic hepatitis B. Quantitative RT-PCR revealed that high levels of mRNA for IL-4 and IFN- gamma correlated with severe inflammation, suggesting that the production of IL-4 and IFN-gamma may be important in tissue injury in the liver in chronic hepatitis B with active viral replication.