HIGH-SPEED DIFFERENTIAL STOPPED-FLOW CALORIMETER

本文介绍了一种初步的单通道、高速、停流式量热计 用于评估混合速度、流速、压降， 热探测灵敏度 该系统采用变速飞轮 以及电致动离合器，以将足够的能量传递到 两个驱动注射器将200微升的试剂体积混合在5 来自驱动注射器的入口管，以及 混合器和检测室保持恒温 在25到50摄氏度之间。 超快热敏电阻（1 ms）位于 检测室，以测量反应期间的温度上升。 一 新的驱动凸轮被使用，它产生恒定的加速度， 注射器 混合室包含石英观察管， 反应的光学测量。 观察管“漂浮”在 O形密封圈可减少混合过程中的冲击载荷。 最新的变化 仪器涉及添加第二个相同的通道， 进行差分测量。

A preliminary single-channel, high-speed, stopped-flow calorimeter has been constructed to evaluate mixing speeds, flow velocities, pressure drops, and thermal detection sensitivity. The system uses a variable-speed flywheel and an electrically actuated clutch to transfer sufficient energy to the two drive syringes to mix reagent volumes of 200 microliters in 5 milliseconds or less. The inlet tubes from the drive syringes, as well as the mixer and the detection chamber, are kept at constant temperature between 25 and 50 deg C. An ultra-fast thermistor (1 ms) is located in the detection chamber to measure the temperature rise during the reaction. A new drive cam is used, which produces a constant acceleration of the syringes. The mixing chamber contains a quartz observation tube to allow optical measurement of the reaction. The observation tube 'floats' in O-ring seals to reduce shock loads during mixing. The latest change to the instrument involved adding a second, identical channel to allow differential measurements to be made.