HIGH-SPEED DIFFERENTIAL STOPPED-FLOW CALORIMETER

高速差示停流热量计

• 批准号: 3874179
• 负责人: C P MUDD
• 金额: --
• 依托单位: NATIONAL CENTER FOR RESEARCH RESOURCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3874179
• 关键词: biomedical equipment development; calorimetry; stop flow technique

A preliminary single-channel, high-speed, stopped-flow calorimeter has been constructed to evaluate mixing speeds, flow velocities, pressure drops, and thermal detection sensitivity. The system uses a variable-speed flywheel and an electrically actuated clutch to transfer sufficient energy to the two drive syringes to mix reagent volumes of 200 microliters in 5 milliseconds or less. The inlet tubes from the drive syringes, as well as the mixer and the detection chamber, are kept at constant temperature between 25 and 50 deg C. An ultra-fast thermistor (1 ms) is located in the detection chamber to measure the temperature rise during the reaction. A new drive cam is used, which produces a constant acceleration of the syringes. The mixing chamber contains a quartz observation tube to allow optical measurement of the reaction. The observation tube 'floats' in O-ring seals to reduce shock loads during mixing. The latest change to the instrument involved adding a second, identical channel to allow differential measurements to be made.

初步的单通道、高速、停流量热仪已经研制成功 用于评估混合速度、流速、压降和 热检测灵敏度。 该系统采用变速飞轮 和一个电动离合器，将足够的能量传递给 两个驱动注射器可将 200 微升试剂混合在 5 个注射器中 毫秒或更短。 驱动注射器的入口管，以及 混合器和检测室保持恒温 25 至 50 摄氏度之间。超快热敏电阻 (1 ms) 位于 检测室测量反应过程中的温升。 一个 使用新的驱动凸轮，产生恒定的加速度 注射器。 混合室包含一个石英观察管，以便 反应的光学测量。 观察管“漂浮”在 O 形圈密封件可减少混合过程中的冲击载荷。 最新更改为 仪器涉及添加第二个相同的通道以允许 进行差分测量。