DEVELOPMENT OF AN EMBRYO CRYOPRESERVATION PROGRAM IN LABORATORY ANIMALS

实验动物胚胎冷冻保存程序的开发

• 批准号: 3916160
• 负责人: P M SCHMIDT
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3916160
• 关键词: cryopreservation; cryoprotective agents; embryo /fetus culture; embryo /fetus transplantation; genetic registry /resource /referral center; in vitro fertilization; laboratory mouse; laboratory rabbit; laboratory rat; reproduction; tissue /organ preservation; tissue resource /registry; veterinary medicine

The program objective is to facilitate the management of the NIH animal Genetic Resource (NIHAGR) through the development and utilization of techniques such as collection, culture, freezing and transfer of embryos and gametes for cryobanking genetic material and improving reproductive potential. The NIHAGR maintains a number of critical species and, within species , a vast number of invaluable genotypes used in biomedical research. Embryo preservation uses a cryoprotectant in the freezing medium which allows sufficient dehydration to protect the blastomeres during freezing. Following thawing and dilution of the cryoprotectant, embryos are cultured to assess in vitro development. Transplantation of embryos into recipient females monitors in vitro fertilization and ova freezing are being examined as techniques to salvage limited genetic material from single females or strains with poor in vitro fertilization rates. Banked embryos are maintained in a frozen state until needed for rederivation. A primary focus of laboratory efforts is the study and optimization of cryobanking technology in mice and rabbits and more recently, in rats. A major research finding is that embryo freezability is confounded and influenced markedly by mouse genotype. Such responses have been examined in over 56 genotypes, most of which display genotype-specific survival rates to standardize embryo freeze-thawing procedures. Ancillary projects are oriented toward the comparative aspects of improving long-term embryo storage with particular emphasis on the study of cryoprotectants and enhancing methods for embryo freezing, thawing, dilution and transfer. Efforts in the rabbit focus on improvements in collection procedures and preservation of embryos.

该计划的目标是促进NIH动物的管理 遗传资源（NIHAGR），通过开发和利用 胚胎的采集、培养、冷冻和移植等技术 和配子来储存遗传物质和提高生殖能力 潜力 NIHAGR保留了一些关键物种， 物种，大量的宝贵的基因型用于生物医学 research. 胚胎保存在冷冻介质中使用冷冻保护剂 这允许足够的脱水来保护卵裂球， 冻死了 解冻和稀释冷冻保护剂后， 进行培养以评估体外发育。 胚胎移植 对受体女性进行体外受精和卵子冷冻监测， 作为一种技术来挽救有限的遗传物质 单个雌性或体外受精率低的品系。 库存 胚胎保持冷冻状态直到需要再衍生。 一 实验室工作的主要重点是研究和优化 在小鼠和兔子以及最近在大鼠身上的冷冻库技术。 一 一项主要的研究发现是胚胎的冷冻能力是混乱的， 受小鼠基因型的显著影响。 对这些反应进行了审查， 在超过56种基因型中，其中大多数表现出基因型特异性存活 标准化胚胎冻融程序。 配套项目 是针对改善长期胚胎的比较方面， 储存，特别强调冷冻保护剂的研究， 用于胚胎冷冻、解冻、稀释和移植的增强方法。 兔子的努力集中在收集程序的改进上， 保存胚胎。