DEVELOPMENT OF AN EMBRYO CRYOPRESERVATION PROGRAM IN LABORATORY ANIMALS

实验动物胚胎冷冻保存程序的开发

• 批准号: 3959914
• 负责人: P M SCHMIDT
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3959914
• 关键词: cryopreservation; cryoprotective agents; embryo /fetus culture; embryo /fetus transplantation; genetic registry /resource /referral center; in vitro fertilization; reproduction; tissue /organ preservation; tissue resource /registry

The program objective is to facilitate the management of the NIH Animal Genetic Resource (NIHAGR) through development and utilization of embryo collection, culture, freezing and transfer as techniques for cryobanking genetic material and improving reproductive potential. The NIHAGR maintains a number of critical species and, within species, a vast number of invaluable genotypes used in biomedical research. A cryoprotectant in the freezing medium allows sufficient dehydration to protect the blastomeres during freezing. Following thawing and dilution of the cryoprotectant, embryos are cultured to assess in vitro development. Transplantation of embryos into recipient females monitors in vitro development and thus biological competence. In vitro fertilization and ova freezing are being examined as techniques to salvage limited genetic material from single females or strains with poor in vivo fertilization rates. Banked embryos are maintained in a frozen state until needed for rederivation. A primary focus of laboratory efforts is the study and optimization of cryobanking technology in mice. A major research finding is that embryo freezability is confounded and influenced markedly by genotype. Such responses have been examined in over 49 mouse genotypes, most of which display genotype-specific survival rates to standardized embryo freeze- thawing procedures. Ancillary projects are oriented toward the comparative aspects of improving long-term embryo storage with particular emphasis on the study of cryoprotectants and enhancing methods for embryo freezing, thawing, dilution and transfer.

该计划的目标是促进NIH的管理 动物遗传资源（NIHAGR）通过开发和 利用胚胎采集、培养、冷冻和移植， 基因材料的冷冻库技术， 生殖潜力 NIHAGR维护了一些 关键物种，在物种内部， 用于生物医学研究的基因型。 冷冻保护剂， 冷冻介质允许充分脱水以保护 冷冻时的卵裂球。 解冻和稀释后， 培养冷冻保护剂、胚胎以在体外评估 发展 将胚胎移植到受体雌性中 监测体外发育，从而监测生物学能力。 在 体外受精和卵子冷冻正在研究中， 技术挽救有限的遗传物质从单一的 雌性或品系体内受精率低。 库存 胚胎保持在冷冻状态，直到需要 再衍生 实验室工作的主要重点是研究 以及小鼠冷冻库技术的优化。 一个主要 研究发现，胚胎的冷冻能力是混乱的， 受基因型影响显著。 这种反应一直是 在超过49种小鼠基因型中进行了检查，其中大多数显示 标准化胚胎冷冻的基因型特异性存活率- 解冻程序。 配套项目面向 改善长期胚胎储存的比较方面， 特别强调对冷冻保护剂的研究， 胚胎冷冻、解冻、稀释和移植的方法。