DEVELOPMENT OF AN EMBRYO CRYOPRESERVATION PROGRAM IN LABORATORY ANIMALS

实验动物胚胎冷冻保存程序的开发

• 批准号: 4705582
• 负责人: P M SCHMIDT
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4705582
• 关键词: cryopreservation; cryoprotective agents; embryo /fetus culture; embryo /fetus transplantation; reproduction

The objective of this program is to facilitate the management of the NIH Animal Genetic Resource (NIH AGR) through development and utilization of embryo collection, culture, freezing and transplantation as techniques for cryobanking genetic stock and improving reproductive potential. Embryos are frozen slowly in medium containing cryoprotectant to allow sufficient dehydration of embryos to insure post-thaw survival. Following dilution of cryoprotectant after thawing, embryos are cultured to assess in vitro development. Embryos are collected and maintained in a frozen state until needed for rederivation of stocks and strains. This reduces genetic drift and serves to safeguard live colonies from catastrophe. Development of this technology allows banking of embryos for long-term storage and subsequent rederivation under the direction of the Geneticist, SAS. Concurrent comparative research studies are designed to identify modifications in methodology which will enhance female response to ovulation induction regimens and improve post-thaw embryo viability.

该计划的目标是促进NIH的管理 动物遗传资源（NIH AGR）通过开发和利用 胚胎收集、培养、冷冻和移植作为 基因库和提高繁殖潜力。 胚胎 在含有冷冻保护剂的培养基中缓慢冷冻， 使胚胎脱水以确保解冻后存活。 稀释后 冷冻保护剂解冻后，培养胚胎以在体外评估 发展 收集胚胎并保持冷冻状态， 需要重新衍生的股票和菌株。 这减少了遗传漂变 保护活着的殖民地免遭灾难 发展 这项技术允许长期储存胚胎， 随后在遗传学家SAS的指导下进行了重新推导。 同时进行的比较研究旨在确定 修改方法，以提高女性对以下问题的反应： 排卵诱导方案和提高解冻后胚胎的活力。