DEVELOPMENT OF AN EMBRYO CRYOPRESERVATION PROGRAM IN LABORATORY ANIMALS

该计划的目标是促进NIH动物的管理 通过胚胎开发和利用的遗传资源（NIHAGR） 收集、培养、冷冻和移植作为 冷冻储存遗传物质和提高繁殖潜力。 的 NIHAGR由几种实验室物种组成， 在小鼠中进行，在兔子中进行。 冷冻银行很混乱 通过在超过30只小鼠中发现的对冷冻保存的独特基因型反应， 基因型研究至今。 胚胎被冷冻在一个可编程的单元中 以确保胚胎存活。 冷冻介质中的冷冻保护剂允许充分脱水， 在冷冻过程中保护卵裂球。 解冻和稀释后， 培养冷冻保护剂胚胎以评估体外发育。 胚胎移植到受体雌性体内监测 发展 体外受精和卵子冷冻正在研究中， 从单身女性身上抢救有限遗传物质技术， 体内受精率低的菌株。 库存胚胎 保持在冻结状态，直到需要重新派生。 并发 比较研究旨在确定 提高女性对诱导排卵反应的方法 方案，提高解冻后胚胎存活率，并检查 基因型对胚胎冷冻保存的影响。

The program objective is to facilitate the management of the NIH Animal Genetic Resource (NIHAGR) through development and utilizaton of embryo collection, culture, freezing and transplantation as techniques for cryobanking genetic material and improving reproductive potential. The NIHAGR consists of several laboratory species for which cryobanking is ongoing in mice and being developed in rabbits. Cryobanking is confounded by unique genotypic responses to cryopreservation found in over 30 mouse genotypes investigated to date. Embryos are frozen in a programable unit at species specific rates to ensure embryo survival. A cryoprotectant in the freezing medium allows sufficient dehydration to protect the blastomeres during freezing. Following thawing and dilution of the cryoprotectant, embryos are cultured to assess in vitro development. Transplantation of embryos into recipient females monitors in vivo development. In vitro fertilization and ova freezing are being examined as tehcniques to salvage limited genetic material from single females or strains with poor in vivo fertilizatiaon rates. Banked embryos are maintained in a frozen state until needed for rederivation. Concurrent comparative research studies are designed to identify modifications in methodology which will enhance female response to ovulation induction regimens, improve post-thaw embryo survival and examine the influence of genotype on embryo cryopreservation.