DEVELOPMENT OF AN EMBRYO CRYOPRESERVATION PROGRAM IN LABORATORY ANIMALS

实验动物胚胎冷冻保存程序的开发

• 批准号: 5204039
• 负责人: P M SCHMIDT
• 金额: --
• 依托单位: NATIONAL CENTER FOR RESEARCH RESOURCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5204039
• 关键词: alternatives to animals in research; cryopreservation; embryo /fetus preservation; genetic registry /resource /referral center; laboratory mouse; laboratory rabbit; laboratory rat; tissue resource /registry; veterinary science

The NIH Animal Genetic Resource (NIHAGR) maintains several animal species and many invaluable genotypes within a species, which are used as models in biomedical research. To protect this germ plasm, an embryo bank was established as an economical means to indefinitely preserve these stocks and strains of rodents and rabbits. Cryopreservation of embryos has other benefits for genotype management such as safeguarding against loss of germ plasm from disease, reproductive failure, natural catastrophe or genetic contamination. The cryobank minimizes changes due to genetic drift by providing a resource from which to periodically rederive defined genotypes for restandardization of foundation breeding colonies. As a recognized repository for laboratory animal species, the NIHAGR routinely provides breeding nuclei upon request throughout the biomedical research community. Rather than shipping live animals, cryopreserved embryos represent a simpler, more humane and easier method to transport requested germ plasm worldwide. Another important impact of embryo handling, cryopreservation, and transfer procedures is to eliminate the vertical transmission of some murine diseases.

NIH动物遗传资源（NIHAGR）保存了几种动物 物种和物种内的许多宝贵的基因型，这是使用 作为生物医学研究的模型。 为了保护这种种质， 建立胚胎库是一种经济的手段， 保存这些啮齿动物和兔子的种群和品系。 胚胎冷冻保存对于基因型管理还有其他好处 例如防止疾病造成的种质损失， 繁殖失败、自然灾害或基因污染。 冷冻库通过提供一个 从其定期重新衍生定义的基因型的资源 重新标准化基础育种群体。 作为公认的 实验室动物物种的储存库，NIHAGR定期 根据要求在整个生物医学 研究社区。 而不是运输活的动物，冷冻保存 胚胎是一种更简单、更人道、更容易的方法， 将所需的种质运送到世界各地。 另一个重要影响是 胚胎处理、冷冻保存和移植程序是为了 消除某些鼠科疾病的垂直传播。