DEVELOPMENT OF AN EMBRYO CRYOPRESERVATION PROGRAM IN LABORATORY ANIMALS

实验动物胚胎冷冻保存程序的开发

• 批准号: 3763311
• 负责人: P M SCHMIDT
• 金额: --
• 依托单位: NATIONAL CENTER FOR RESEARCH RESOURCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3763311
• 关键词: alternatives to animals in research; cryopreservation; embryo /fetus preservation; genetic registry /resource /referral center; genotype; laboratory mouse; laboratory rabbit; laboratory rat; tissue resource /registry; veterinary science

The NIH Animal Genetic Resource (NIHAGR) maintains a large number of animal species and many invaluable genotypes within a species used as models in biomedical research. To protect this germ plasm, an embryo bank was established as an economical means to indefinitely preserve these stocks and strains of rodents and rabbits. Cryopreservation of embryos has other benefits for genotype management such as safeguarding against loss of germ plasm from disease, reproductive failure, natural catastrophe or genetic contamination. The cryobank minimizes changes due to genetic drift by providing a resource from which to periodically rederive defined genotypes for restandardization of foundation breeding colonies. As a recognized repository for laboratory animal species, the NIHAGR routinely provides breeding nuclei upon request throughout the biomedical research community. Rather than shipping live animals, cryopreserved embryos represent a simpler, more humane and easier method to transport requested germ plasm worldwide. Another important impact of embryo handling, cryopreservation, and transfer procedures is to eliminate the vertical transmission of some murine diseases.

美国国立卫生研究院动物遗传资源（NIHAGR）保存了大量的 动物物种和一个物种中许多宝贵的基因型， 生物医学研究中的模型。 为了保护这种种质， 建立银行是为了无限期地保存 这些啮齿动物和兔子的种群和品系。 冷冻保存 胚胎对基因型管理还有其他好处， 防止疾病、生殖失败、自然 灾难或基因污染。 冷冻库将由于 通过提供一种资源， 重新推导定义的基因型，以重新标准化基础育种 殖民地 作为一个公认的实验室动物物种库， NIHAGR在整个研究期间根据要求定期提供育种细胞核。 生物医学研究社区。 而不是运送活的动物， 冷冻胚胎是一种更简单、更人道、更容易的方法， 将所需的种质运送到世界各地 另一个重要影响 胚胎处理、冷冻保存和移植程序的目的是 消除某些鼠科疾病的垂直传播。