DEVELOPMENT OF AN EMBRYO CRYOPRESERVATION PROGRAM IN LABORATORY ANIMALS

实验动物胚胎冷冻保存程序的开发

• 批准号: 3785373
• 负责人: P M SCHMIDT
• 金额: --
• 依托单位: NATIONAL CENTER FOR RESEARCH RESOURCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3785373
• 关键词: alternatives to animals in research; animal breeding; cryopreservation; embryo /fetus preservation; genetic registry /resource /referral center; genotype; laboratory mouse; laboratory rabbit; laboratory rat; tissue resource /registry; veterinary science

The NIH Animal Genetic Resource (NIHAGR) maintains a large number of animal species and many invaluable genotypes within a species used as models in biomedical research. To protect this germ plasm, an embryo bank was established as an economical means to indefinitely preserve these stocks and strains of rodents and rabbits. Cryopreservation of embryos has other benefits for genotype management such as safeguarding against loss of germ plasm from disease, reproductive failure, natural catastrophe or genetic contamination. The cryobank minimizes changes due to genetic drift by providing a resource from which to periodically rederive defined genotypes for restandardization of foundation breeding colonies. As a recognized repository for laboratory animal species, the NIHAGR routinely provides breeding nuclei upon request throughout the biomedical research community. Rather than shipping live animals, cryopreserved embryos represent a simpler, more humane and easier method to transport requested germ plasm worldwide. Another important impact of embryo handling, cryopreservation, and transfer procedures is to eliminate the vertical transmission of some murine diseases.

美国国立卫生研究院动物遗传资源（NIHAGR）维护了大量的