DEVELOPMENT OF AN EMBRYO CRYOPRESERVATION PROGRAM IN LABORATORY ANIMALS

美国国立卫生研究院动物遗传资源(NIHAGR)保存了大量 动物物种和物种内许多宝贵的基因类型被用作 生物医学研究中的模型。为了保护这个种质，一个胚胎 银行的成立是作为一种经济手段无限期地保存 这些种群和品系的啮齿动物和兔子。超低温保存 胚胎对基因管理还有其他好处，如保护 防止因疾病、生殖失败而造成的种质损失，天然的 灾难或基因污染。低温数据库最大限度地减少了因此而产生的更改 通过提供一种资源来周期性地 为基础育种重新标准化重新衍生已确定的基因类型 殖民地。作为公认的实验动物物种保存库， NIHAGR根据要求在整个 生物医学研究社区。而不是运输活的动物， 冷冻胚胎代表了一种更简单、更人道和更容易的方法 将所需的种质运送到世界各地。另一个重要影响 胚胎处理、冷冻保存和移植程序的关键是 消除一些鼠疫的垂直传播。

The NIH Animal Genetic Resource (NIHAGR) maintains a large number of animal species and many invaluable genotypes within a species used as models in biomedical research. To protect this germ plasm, an embryo bank was established as an economical means to indefinitely preserve these stocks and strains of rodents and rabbits. Cryopreservation of embryos has other benefits for genotype management such as safeguarding against loss of germ plasm from disease, reproductive failure, natural catastrophe or genetic contamination. The cryobank minimizes changes due to genetic drift by providing a resource from which to periodically rederive defined genotypes for restandardization of foundation breeding colonies. As a recognized repository for laboratory animal species, the NIHAGR routinely provides breeding nuclei upon request throughout the biomedical research community. Rather than shipping live animals, cryopreserved embryos represent a simpler, more humane and easier method to transport requested germ plasm worldwide. Another important impact of embryo handling, cryopreservation, and transfer procedures is to eliminate the vertical transmission of some murine diseases.