INTERACTION BETWEEN CEREBRAL CAPILLARY ENDOTHELIUM AND IMMUNE CELLS

这项研究代表了一项持续的努力，以发展和建立 最可靠和可重复的培养方法 来源于SJL小鼠的微血管内皮细胞 到EAE。 几种不同的分离、解离程序 并评估细胞的接种以获得最大的 内皮的屈服。 这些植物的生长和繁殖 在存在或不存在内皮细胞的情况下测定细胞 生长因子（ECGF）。 这些研究虽然不完整，但表明小鼠 内皮细胞需要纤连蛋白基质和ECGF 补充培养基以获得最佳的体外生长和繁殖。 无论细胞产量如何，每个测试程序 不干扰因子VIII的表达。 感应 I-A+抗原和内皮细胞对 IFN γ在所有内皮培养物中都是可能的。 因此 建立的鼠EC培养物适合于设计模型 系统，以研究脑EC和 免疫细胞在生活状态，这是重要的， 了解几种神经系统疾病的发病机制 紊乱

This study represents a continuous effort to develop and establish the most reliable and reproducible method for culturing cerebromicrovascular endothelium derived from SJL mice susceptible to EAE. Several different procedures of isolation, dissociation and seeding of the cells were evaluated to obtain the greatest yield of the endothelium. The growth and propagation of these cells was determined in the presence or absence of endothelial cell growth factor (ECGF). These investigations, although incomplete indicate that the murine endothelial cells require a fibronectin matrix and ECGF supplemented medium for the best growth and propagation in vitro. Each of the tested procedures irrespective o the cellular yield does not interfere with the expression of Factor VIII. Induction of I-A+ antigen and proliferating response of the endothelium to IFNgamma is possible in all endothelial cultures. Thus the established murine EC cultures are suitable for designing a model system to investigate the interaction between the cerebral EC and immune cells in living state, which are of importance in the understanding of the pathogenesis of several neurological disorders.