STUDIES OF IMMUNOGLOBULIN GENE REARRANGEMENT

免疫球蛋白基因重排的研究

• 批准号: 3940666
• 负责人: M GELLERT
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3940666
• 关键词: B lymphocyte; antibody formation; bacterial DNA; bioassay; gene expression; genetic manipulation; genetic recombination; immunoglobulins; molecular biology; nucleic acid sequence; plasmids; tissue /cell culture

The level of immunoglobulin gene V-J recombination activity in cell lines derived from lymphoid or nonlymphoid lineages was examined. The assay uses an extrachromosomal DNA substrate, and thus avoids difficulties associated with the use of chromosomally integrated DNA. The recombination activity is found to vary progressively during B lymphoid development. It is low at a very early stage (pro-GMB), much higher in pro-B cells, but then declines through later stages, reaching an undetectable level in mature B cells. The activity is also present in multi potential progenitors of myeloid cells, and in pre-T but not mature T cells. No activity was found in several nonhematopoietic cell lines.

免疫球蛋白基因V-J重组活性水平 来自淋巴或非淋巴谱系的细胞系是 考察 该测定使用染色体外DNA底物， 从而避免了与使用 染色体整合的DNA 重组活性是 发现在B淋巴发育过程中进行性变化。 是 在非常早期阶段低（pro-GMB），在pro-B细胞中高得多， 但在后期会下降， 成熟B细胞水平。 该活动也存在于多个 骨髓细胞的潜在祖细胞，在前T细胞，但不是 成熟T细胞 在几个国家没有发现活动。 非造血细胞系