STUDIES OF IMMUNOGLOBULIN GENE REARRANGEMENT
STUDIES OF IMMUNOGLOBULIN GENE REARRANGEMENT
批准号:
2573012
负责人:
M GELLERT
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$0.0万
依托单位国家:
美国
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--
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美国
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中文摘要
V(D)J重组过程,
免疫球蛋白和T细胞受体基因在淋巴细胞中的表达,
对产生免疫反应的多样性至关重要。 的
现在已知反应分两个阶段进行。 在第一阶段中,
在靶位点产生特异性双链断裂。端部
这些断裂处的DNA编码序列总是连接在一起,
DNA发夹 根据细胞实验,
已知一种不寻常的裂解类型利用RAG 1的产物
和RAG 2基因,但尚不清楚它们如何发挥作用。 后期
重组的阶段不那么具体;最终的结合产物
是利用许多因素也参与修复
辐射损伤 我们现在已经重现了第一次裂解反应
在生化定义的反应中,仅需要纯化的RAG-1
和RAG-2蛋白。 含有识别信号的DNA片段
切割一个发夹序列(RSS)以产生一个发夹编码末端和一个钝信号
就像在体内一样。 此外,在要求更高的反应中,
条件下,切割需要一对不同类型的RSS
是体内重组所必需的。 因此,RAG蛋白
单独包含V(D)J特异性的所有信息
重组 我们还发现,发夹是在一个
反应在化学上与转座重组非常相似。一个
V(D)J重组与转座的进化关系
元素之前已经提出,我们的结果提供了
迄今为止最有力的数据支持了这一观点。
英文摘要
The V(D)J recombination process that assembles functional
immunoglobulin and T cell receptor genes in lymphoid cells is
essential for generating the diversity of the immune response. The
reaction is now known to occur in two stages. In the first stage,
specific double-strand breaks are made at the target sites. The ends
of the coding DNA sequence at these breaks are always joined back on
themselves as DNA hairpins. Based on cellular experiments, this
unusual type of cleavage was known to utilize the products of the RAG1
and RAG2 genes, but it was not clear how they functioned. The later
stages of recombination are less specific; the final joined products
are made with the use of many factors also involved in the repair of
radiation damage. We have now reproduced the first cleavage reaction
in a biochemically defined reaction requiring only the purified RAG-1
and RAG-2 proteins. A DNA fragment containing the recognition signal
sequence (RSS) is cut to make a hairpin coding end and a blunt signal
end, just as in vivo. Furthermore, in more demanding reaction
conditions, cleavage requires a pair of RSSs of the different types
that are needed for recombination in vivo. Thus the RAG proteins
alone contain all the information for the specificity of V(D)J
recombination. We have also found that the hairpins are made in a
reaction very similar chemically to transpositional recombination. An
evolutionary relation between V(D)J recombination and transposable
elements had previously been suggested, and our results provide the
strongest data so far in support of this idea.
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EFFECTS OF DNA SUPERCOILING ON THE TOPOLOGICAL PROPERTIES OF NUCLEOSOMES
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批准号:3897281
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负责人:M GELLERT
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EFFECTS OF DNA SUPERCOILING ON THE TOPOLOGICAL PROPERTIES OF NUCLEOSOMES
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批准号:3940667
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负责人:M GELLERT
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依托单位:
STUDIES OF IMMUNOGLOBULIN GENE REARRANGEMENT
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批准号:3917766
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EFFECTS OF DNA SUPERCOILING ON THE TOPOLOGICAL PROPERTIES OF NUCLEOSOMES
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批准号:3917767
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负责人:M GELLERT
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STUDIES OF IMMUNOGLOBULIN GENE REARRANGEMENT
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批准号:3776322
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STUDIES OF FUNCTIONS INVOLVED IN GENETIC RECOMBINATION
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批准号:3754209
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STUDIES OF FUNCTIONS INVOLVED IN GENETIC RECOMBINATION
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批准号:6161956
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STUDIES OF IMMUNOGLOBULIN GENE REARRANGEMENT
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STUDIES OF FUNCTIONS INVOLVED IN GENETIC RECOMBINATION
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批准号:5201974
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STUDIES OF IMMUNOGLOBULIN GENE REARRANGEMENT
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STUDY OF FUNCTIONS INVOLVED IN GENETIC RECOMBINATION
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STUDY OF FUNCTIONS INVOLVED IN GENETIC RECOMBINATION
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STUDIES OF IMMUNOGLOBULIN GENE REARRANGEMENT
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STUDIES OF IMMUNOGLOBULIN GENE REARRANGEMENT
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批准号:3964502
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STUDIES OF IMMUNOGLOBULIN GENE REARRANGEMENT
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批准号:3754210
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STUDIES OF IMMUNOGLOBULIN GENE REARRANGEMENT
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批准号:3839883
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STUDIES OF FUNCTIONS INVOLVED IN GENETIC RECOMBINATION
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批准号:3854834
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STUDIES OF FUNCTIONS INVOLVED IN GENETIC RECOMBINATION
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批准号:6105239
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STUDY OF FUNCTIONS INVOLVED IN GENETIC RECOMBINATION
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批准号:3917765
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STUDIES OF IMMUNOGLOBULIN GENE REARRANGEMENT
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