KINETICS OF A NEW ANTI-FUNGAL AGENT

新型抗真菌剂的动力学

• 批准号: 3963151
• 负责人: P K NARANG
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3963151
• 关键词: analytical method; antifungal agents; blood chemistry; drug metabolism; high performance liquid chromatography; human tissue; microorganism disease chemotherapy; pharmacokinetics

A new HPLC method to quantitate SF 86,327 (an antifungal compound from Sandoz) was developed, that eliminated extremely lengthy extraction steps. We were able to validate our method by running 4 standard curves with an overall variability of 5%. We have been able to analyze patient plasma samples (a total of about 60) which were also analyzed using a microbiological procedure. Correlation analysis of these data indicate that the HPLC procedure is more specific as the slope of the correlation line (microbio. vs. HPLC) was approximately 1.38. The results, that microbiological assay may not be specific for the parent compound but may also be measuring an active metabolite, are of interest and have possible clinical implication in drug dosing.

高效液相色谱法定量测定SF 86,327(一种植物抗真菌化合物 Sandoz)被开发出来，消除了极其冗长的提取步骤。 我们通过运行4条标准曲线来验证我们的方法 总体变异性为5%。我们已经能够分析患者的血浆 样本(总共约60个)也用 微生物程序。对这些数据的相关性分析表明 当相关系数的斜率越大时，高效液相程序越具体 Line(微生物。与高效液相色谱相比)约为1.38。结果，就是 微生物检测可能不是针对母体化合物的，但可以 也在测量一种活性代谢物，是有兴趣的和有可能的 药物剂量的临床意义。