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CORE--CELL SORTING UNIT

CORE--CELL SORTING UNIT
核心--细胞分选单元
批准号:
5206811
负责人:
ROSS S BASCH
金额:
$0.0万
依托单位:
--
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:

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中文摘要
翻译
细胞分选单元提供最先进的流动技术 为卡普兰综合癌症中心成员提供服务和培训。这些 服务旨在促进成员的研究,教育 他们对流动技术提供的技术可能性和 培训博士后和博士后使用这项技术。 P30CA160879010 转基因小鼠研究机构为卡普兰提供了资源 综合癌症中心成员寻求培育转基因小鼠 研究基因调控的各个方面。资源供应的使用者 以适合注入的方式制备的DNA构建体 受精卵。潜在的创始动物被生产出来,并且 由资源设施移除并提供给研究的尾巴 用于DNA分析的调查者通过Southern blotting确定 整合阳性的小鼠。整合呈阳性的动物有 然后交给个人调查人员来繁育和研究 注射的DNA构建体表达的影响。
英文摘要
The Cell Sorting Unit makes available state-of-the-art flow technology service and training to Kaplan Comprehensive Cancer Center members. These services are designed to facilitate the research of the member, educate them about the technological possibilities offered by flow techniques and train pre- and post-doctoral students in the use of this technology. P30CA160879010 The Transgenic Mouse Research Facility provides a resource for Kaplan Comprehensive Cancer Center members seeking to generate transgenic mice to study various aspects of gene regulation. Users of the resource supply DNA constructs prepared in a manner suitable for injection into fertilized mouse eggs. Potential founder animals are produced and the tails removed by the resource facility and provided to the research investigator for DNA analysis by Southern blotting to determine integration-positive mice. Animals which are integration-positive are then handed over to the individual investigators to breed and study the effect of the expression of the injected DNA construct.
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C21, a Transcriptional Regulator in Hematopoiesis
C21, a Transcriptional Regulator in Hematopoiesis
C21, a Transcriptional Regulator in Hematopoiesis
C21, a Transcriptional Regulator in Hematopoiesis
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