XRAY CRYOGENIC PRESSURE CELL & DELIVERY SYSTEM FOR PREPARATION OF XE DERIVATIVES

X 射线低温压力盒

• 批准号: 6119334
• 负责人: S M SOLTIS
• 金额: --
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119334
• 关键词: proteins; technology /technique

Methods for preparing cryo-cooled xenon-derivatized protein crystals have been further developed. SSRL staff designed new tools and developed procedures for easy manipulation of the sample crystal. Samples are first pressurized and then quickly flash-cooled in liquid nitrogen. Two systems based on the original SSRL-CalTech prototype have been fabricated and installed on BL7-1 and BL9-1. Instructions for their use have been documented on the WorldWideWeb site. In addition, a workshop was held (see separate D subproject) which included a lecture as well as hands on training for the preparation of xenon derivatives. Participants of the workshop, as well as other users of the system, have found it simple and safe to use.

制备低温冷却的氙衍生蛋白的方法 晶体得到了进一步的发展。 SSRL工作人员设计了新工具 并开发了易于操作样品晶体的程序。 样品首先加压，然后在液体中快速快速冷却 氮气 两个基于SSRL-CalTech原型的系统 已制造并安装在BL 7 -1和BL 9 -1上。 指令 已在万维网网站上记录。 在 此外，还举办了一个讲习班（见单独的D分项目）， 包括一个讲座，以及实践培训，为准备 氙衍生物 研讨会的与会者以及其他 该系统的用户发现它使用起来既简单又安全。