IN-SITU UV/VIS SPECTROSCOPY AT HELIUM TEMPERATURES

氦气温度下的原位紫外/可见光谱

• 批准号: 8169997
• 负责人: S M SOLTIS
• 金额: $ 3.39万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169997
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Data; Fees; Funding; Grant; Helium; In Situ; Institution; Laboratories; Measurement; Methods; Research; Research Personnel; Resolution; Resources; Robotics; Source; Spectrum Analysis; System; Temperature; United States National Institutes of Health; X ray diffraction analysis; X-Ray Diffraction; absorption; base; cold temperature; cytochrome ba3

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Measurement of the auto-reduction rate by the x-ray beam was continued for the oxidized form of cytochrome ba3. X-ray diffraction data and single crystal absorption spectra in the UV/Visible region were recorded at ~15 K. Based on these measurements and previous measuremetns, the rate of reduction was reduced by a factor of 2-3 at the lower temperature. Approximetely 2-4 frames could be collected from a single crystal that diffracted to ~3.0 A. Typically, only 1 out of 15 crystals diffract to this resolution requiring approximtely 90/3 X 15 = 450 crystals. Methods to produce a higher yield of good diffracting crystals will be caried out in J. Fee?s laboratory and compaibility of the UV/VIS spectroscopy system and the robotic monting system for highthroughput screeening will be developed at SSRL.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 继续对氧化形式的细胞色素Ba3进行X射线自动还原速率的测量。在~15K记录了X-射线衍射数据和紫外/可见光区域的单晶吸收光谱，根据这些测量和以前的测量，在较低的温度下，还原速度降低了2-3倍。大约2-4帧可以从衍射到~3.0A的单个晶体中收集到。典型地，15个晶体中只有1个衍射到这个分辨率需要大约90/3×15=450个晶体。J·菲？S实验室将研究生产高质量衍射晶体的方法，SSRL将开发与UV/VIS光谱系统和用于高通量筛选的机器人蒙太奇系统的兼容性。