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STRUCTURE DETERMINATION OF A MEMBRANE PROTEIN USING SULFUR SAD

STRUCTURE DETERMINATION OF A MEMBRANE PROTEIN USING SULFUR SAD
使用硫 SAD 测定膜蛋白的结构
批准号:
8169923
负责人:
S M SOLTIS
金额:
$0.92万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2010
资助国家:
美国
项目状态:
已结题
起止时间:
2010-05-01 至 2011-02-28

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中文摘要
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英文摘要
This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. An extracellular membrane protein failed to incorporate Se and other heavy atoms for structure determination. A strategy was developed to collect S data for structure determination (the S K-edge is at 5.015 A). Data were recorded at a wavelength of 2 A. A helium cold stream was incorporated to reduce background scatter. Highly redundant data were collected yielding a redundancy of 22 (3 positions on the crystal, 360 degrees of data each, 1080 degrees total) yielding an Rsym of 10% to 2.1 A resolution. Images were collected in an inverse beam mode using a wedge of 1 image (for every 0.5 deg. oscillation, phi was rotated 180 degrees). The data will be analyzed to establish the structure and the relevance of the method.
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MITIGATION OF RADIATION DAMAGE IN MACROMOLECULAR CRYSTALLOGRAPHY EXPERIMENTS
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    8362434
  • 项目类别:
  • 资助金额:
    $0.27万
  • 财政年份:
    2011
  • 负责人:
    S M SOLTIS
  • 依托单位:
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  • 项目类别:
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  • 财政年份:
    2011
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  • 项目类别:
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  • 财政年份:
    2011
  • 负责人:
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国内基金
海外基金
Scalable Learning and Optimization: High-dimensional Models and Online Decision-Making Strategies for Big Data Analysis
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Development of a Linear Stochastic Model for Wind Field Reconstruction from Limited Measurement Data
  • 批准号:
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  • 项目类别:
    --
  • 资助金额:
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    2020
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  • 依托单位:
基于Linked Open Data的Web服务语义互操作关键技术
  • 批准号:
    61373035
  • 项目类别:
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  • 资助金额:
    77.0万元
  • 批准年份:
    2013
  • 负责人:
    冯志勇
  • 依托单位: