IN-SITU UV/VIS SPECTROSCOPY AT HELIUM TEMPERATURES

氦气温度下的原位紫外/可见光谱

• 批准号: 7954285
• 负责人: S M SOLTIS
• 金额: $ 1.24万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954285
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Data; Enzymes; Funding; Grant; Helium; In Situ; Institution; Liquid substance; Measurement; Measures; Metals; Nitrogen; Oxidation-Reduction; Radiation; Research; Research Personnel; Resources; Site; Source; Spectrum Analysis; Stream; System; Temperature; United States National Institutes of Health; absorption; base; cold temperature; research study; structural biology; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The simultaneous acquisition of x-ray diffraction data and single crystal absorption spectra in the UV/Visible region was explored at near liguid helium temperatures. A single crystal microspectrophotometer was set up on BL12-2 to record spectra ?in-situ? from crystals mounted on the goniometer. The conventional liquid nitrogen cold stream was replaced by a liquie helium coldstream system. During diffraction experiments, spectroscopic measurements were analyzed to confirm the redox state of the metal site of the enzyme crystal. Rates of radiation-induced photo-reduction were measured at 80 K and ~15 K. Based on these measurements, the rate of reduction was reduced by a factor of 2-3 at the lower temperature.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 在接近液氦的温度下探索了同时采集 X 射线衍射数据和紫外/可见光区域的单晶吸收光谱。 在 BL12-2 上安装单晶显微分光光度计来记录“原位”光谱。来自安装在测角仪上的晶体。 传统的液氮冷流被液氦冷流系统取代。 在衍射实验中，分析光谱测量以确认酶晶体金属位点的氧化还原状态。 在 80 K 和 ~15 K 下测量了辐射引起的光还原率。根据这些测量结果，在较低温度下还原率降低了 2-3 倍。