IN-SITU UV/VIS SPECTROSCOPY AT HELIUM TEMPERATURES

氦气温度下的原位紫外/可见光谱

• 批准号: 7954285
• 负责人: S M SOLTIS
• 金额: $ 1.24万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954285
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Data; Enzymes; Funding; Grant; Helium; In Situ; Institution; Liquid substance; Measurement; Measures; Metals; Nitrogen; Oxidation-Reduction; Radiation; Research; Research Personnel; Resources; Site; Source; Spectrum Analysis; Stream; System; Temperature; United States National Institutes of Health; absorption; base; cold temperature; research study; structural biology; synchrotron radiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The simultaneous acquisition of x-ray diffraction data and single crystal absorption spectra in the UV/Visible region was explored at near liguid helium temperatures. A single crystal microspectrophotometer was set up on BL12-2 to record spectra ?in-situ? from crystals mounted on the goniometer. The conventional liquid nitrogen cold stream was replaced by a liquie helium coldstream system. During diffraction experiments, spectroscopic measurements were analyzed to confirm the redox state of the metal site of the enzyme crystal. Rates of radiation-induced photo-reduction were measured at 80 K and ~15 K. Based on these measurements, the rate of reduction was reduced by a factor of 2-3 at the lower temperature.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在近距离氦气温度下，探索了紫外线/可见区域中X射线衍射数据和单晶吸收光谱的同时采集。 在BL12-2上设置了单个晶体微光光学计，以记录光谱吗？从晶体安装在角度计上。 传统的液氮冷水被液化氦冷路系统取代。 在衍射实验中，分析光谱测量值以确认酶晶体的金属位点的氧化还原状态。 在80 K和〜15 K处测量了辐射诱导的摄影速率。根据这些测量值，在较低温度下，还原率降低了2-3倍。